CAJ | 학술논문

目的筛选特异性流感病毒H3N2抗原模拟表位,为开展新的流感病毒疫苗研究探索新的途径.方法应用噬菌体表面展示技术,以抗-H3N2的单克隆抗体作为固相筛选分子,对人工合成的噬菌体随机肽库进行5轮"吸附-洗脱-扩增"的筛选过程,在第5轮筛选后,随机挑取48个克隆,经噬菌体酶联免疫吸附法(ELISA)鉴定并进行交叉反应实验以及竞争抑制性实验,最后对所选克隆进行DNA序列分析,以确定H3N2抗原的模拟表位.结果经噬菌体富集后,从随机筛选的48个克隆中得到21个阳性克隆,经ELISA鉴定及交叉反应实验、竞争抑制性实验后,确定氨基酸序列XTXPYXX为H3N2的模拟表位.结论用噬菌体7肽库筛选得到H3N2的模拟表位,为开展用流感病毒模拟表位探索新的防治方法研究奠定了基础.
목적 사선특이성류감병독H3N2항원모의표위,위개전신적류감병독역묘연구탐색신적도경.방법 응용서균체표면전시기술,이항-H3N2적단극륭항체작위고상사선분자,대인공합성적서균체수궤태고진행5륜"흡부-세탈-확증"적사선과정,재제5륜사선후,수궤도취48개극륭,경서균체매련면역흡부법(ELISA)감정병진행교차반응실험이급경쟁억제성실험,최후대소선극륭진행DNA서렬분석,이학정H3N2항원적모의표위.결과 경서균체부집후,종수궤사선적48개극륭중득도21개양성극륭,경ELISA감정급교차반응실험、경쟁억제성실험후,학정안기산서렬XTXPYXX위H3N2적모의표위.결론 용서균체7태고사선득도H3N2적모의표위,위개전용류감병독모의표위탐색신적방치방법연구전정료기출.
Objective To screen the influenza A (H3N2) mimotopes by using phage display library. Methods Using influenza A (H3N2) monaclonai antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. Results 21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2). Conclusion Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza vitals vaccine development.