中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
4期
254-259
,共6页
李强翔%钟惠菊%龚汉仁%朱飞跃%王琳娜%申道君%李果%王彩云%秦成生
李彊翔%鐘惠菊%龔漢仁%硃飛躍%王琳娜%申道君%李果%王綵雲%秦成生
리강상%종혜국%공한인%주비약%왕림나%신도군%리과%왕채운%진성생
葛根素%糖尿病肾病%动物,实验%胶原%抗坏血酸%复方合剂
葛根素%糖尿病腎病%動物,實驗%膠原%抗壞血痠%複方閤劑
갈근소%당뇨병신병%동물,실험%효원%항배혈산%복방합제
PUERARIN%Diabetic nephropathies%Animals,laboratory%Collagen%Ascorbic acid%Drug combinations
目的 观察复方葛根素对糖尿病大鼠肾小球Ⅳ型胶原(IV-C)表达的影响.方法 利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠按照数字表法随机分为正常对照组(10只)、糖尿病组(10只)、葛根素治疗组(10只)、维生素C治疗组(10只)、维生素C和葛根素联合治疗组(10只).治疗12周,观察治疗期间及治疗后大鼠的一般状况、血糖、尿素氮、血肌酐,内生肌酐清除率、糖化血红蛋白、血清Ⅳ-C、24 h尿蛋白排泄率,免疫组化检测肾组织Ⅳ-C蛋白表达,原位杂交检测肾组织Ⅳ-Cα1基因表达.结果 (1)4个糖尿病大鼠模型组大鼠均出现肾脏功能损害;(2)葛根素和维生素C能改善基本状况,降低糖尿病大鼠(葛根素治疗组、维生素C治疗组、联合治疗组)的尿素氮[(8.68±0.43)、(7.98±0.47)和(5.76±0.82)μmol/L]、血肌酐[(74.68±8.20)、(75.52±7.98)和(58.66±6.65)mmol/L]、24 h尿白蛋白排泄率[(18.40±0.37)、(17.24±0.30)和(9.97±1.27)mg/24 h×10-3];增加内生肌酐清除率[(0.59±0.21)、(0.61±0.14)和(0.69±0.32)ml/min];Ⅳ-C、[(111.56±14.61),吸光度显著下调(110.78±9.69)和(95.44±9.97)].结论 复方葛根素可能通过抑制Ⅳ-C的表达,具有确切的肾脏保护作用.
目的 觀察複方葛根素對糖尿病大鼠腎小毬Ⅳ型膠原(IV-C)錶達的影響.方法 利用鏈脲佐菌素腹腔註射法誘導建立1型糖尿病大鼠模型,將實驗用SD大鼠按照數字錶法隨機分為正常對照組(10隻)、糖尿病組(10隻)、葛根素治療組(10隻)、維生素C治療組(10隻)、維生素C和葛根素聯閤治療組(10隻).治療12週,觀察治療期間及治療後大鼠的一般狀況、血糖、尿素氮、血肌酐,內生肌酐清除率、糖化血紅蛋白、血清Ⅳ-C、24 h尿蛋白排洩率,免疫組化檢測腎組織Ⅳ-C蛋白錶達,原位雜交檢測腎組織Ⅳ-Cα1基因錶達.結果 (1)4箇糖尿病大鼠模型組大鼠均齣現腎髒功能損害;(2)葛根素和維生素C能改善基本狀況,降低糖尿病大鼠(葛根素治療組、維生素C治療組、聯閤治療組)的尿素氮[(8.68±0.43)、(7.98±0.47)和(5.76±0.82)μmol/L]、血肌酐[(74.68±8.20)、(75.52±7.98)和(58.66±6.65)mmol/L]、24 h尿白蛋白排洩率[(18.40±0.37)、(17.24±0.30)和(9.97±1.27)mg/24 h×10-3];增加內生肌酐清除率[(0.59±0.21)、(0.61±0.14)和(0.69±0.32)ml/min];Ⅳ-C、[(111.56±14.61),吸光度顯著下調(110.78±9.69)和(95.44±9.97)].結論 複方葛根素可能通過抑製Ⅳ-C的錶達,具有確切的腎髒保護作用.
목적 관찰복방갈근소대당뇨병대서신소구Ⅳ형효원(IV-C)표체적영향.방법 이용련뇨좌균소복강주사법유도건립1형당뇨병대서모형,장실험용SD대서안조수자표법수궤분위정상대조조(10지)、당뇨병조(10지)、갈근소치료조(10지)、유생소C치료조(10지)、유생소C화갈근소연합치료조(10지).치료12주,관찰치료기간급치료후대서적일반상황、혈당、뇨소담、혈기항,내생기항청제솔、당화혈홍단백、혈청Ⅳ-C、24 h뇨단백배설솔,면역조화검측신조직Ⅳ-C단백표체,원위잡교검측신조직Ⅳ-Cα1기인표체.결과 (1)4개당뇨병대서모형조대서균출현신장공능손해;(2)갈근소화유생소C능개선기본상황,강저당뇨병대서(갈근소치료조、유생소C치료조、연합치료조)적뇨소담[(8.68±0.43)、(7.98±0.47)화(5.76±0.82)μmol/L]、혈기항[(74.68±8.20)、(75.52±7.98)화(58.66±6.65)mmol/L]、24 h뇨백단백배설솔[(18.40±0.37)、(17.24±0.30)화(9.97±1.27)mg/24 h×10-3];증가내생기항청제솔[(0.59±0.21)、(0.61±0.14)화(0.69±0.32)ml/min];Ⅳ-C、[(111.56±14.61),흡광도현저하조(110.78±9.69)화(95.44±9.97)].결론 복방갈근소가능통과억제Ⅳ-C적표체,구유학절적신장보호작용.
Objective To observe the effect of compound Puerarin on collagen IV of streptozotocininduced diabetic rats.Methods Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin(STZ).Rats were allocated randomly to control group(10),diabetes model group(10),Vitamin C group(10),Puerarin group(10),vitamin C plus Puerarin group(10).The S tudy period lasted for 12 weeks.During and after the treatment,the general state,blood glucose levels,glycosylated hemoglobin,blood urea nitrogen,serum collagen IV,blood urea nitrogen,serum creatinine,urinary albumin excretion rate of the 24-hour,and clearance rate of creatinine collagen IV protein were determined by immunohistochemistoche analysis as well as type the gene expression of collagen IVα1 mRNA were determined by in situ hybridization analysis in the kidney tissue of different groups.Results (1)Diabetes mellitus and renal function lesion occurred in the four groups.(2)Vitamin C and Puerarin could improve the general conditions of diabetic Rats,decrease blood urea nitrogen[(8.68±0.43),(7.98±0.47)and (5.76±0.82)umol/L,serum creatinine[(74.68±8.20),(75.52±7.98)and(58.66±6.65)mmol/L],and urinary albumin excretion rate of the 24-hour[(18.40±0.37),(17.24±0.30)and(9.97±1.27)mg/24 h×10-3];increase clearance rate of creatinine[(0.59±0.21),(0.61±0.14)and(0.69±0.32)ml/min],the expression of collage IV absorbance[(111.56±14.61),(110.78±9.69)and(95.44±9.97)]in the diabetic Rats were significantly inhibited at the same time.Conclusion The compound Puerarin might have some functions on preventing ren by inhibiting expression of type IV collagen.
