CAJ | 학술논문

目的研究幽门螺杆菌(H.pylori)cagA vacA基因亚型及其与抗生素耐药的关系.方法用琼脂稀释法测定50例H.pylori临床分离株对克拉霉素,阿莫西林,替硝唑,甲硝唑的最低抑菌浓度(MIC),并计算MIC50和MIC90值.用多重PCR鉴定H.pylori菌株的vacA(s1a,s1b,s2,m1,m2)及cagA的型别.结果克拉霉素,阿莫西林,替硝唑,甲硝唑对H.pylori的MIC50和MIC90分别为:＜0.016 mg@L-1,0.016 mg@L-1;0.016mg@L-1,0.047 mg@L-1;16.000 mg@L-1,52.000 mg@L-1;25.140mg@L-1,76.800mg@L-1.H.pylori菌株对克拉霉素,阿莫西林,替硝唑,甲硝唑的耐药率分别为0%,0%,20%和38%.在对替硝唑敏感的H.pylori菌株中,vacA s1a,s1b和s2型分别占50.0%,42.5%和7.5%;vacA m1和m2型占60.0%和40.0%;vacA sla/m1,s1b/m1,s1a/m2,s1b/m2和s2/m2型分别占32.5%,27.5%,17.5%,15.0%和12.5%;cagA+和cagA-型为87.5%和12.5%.在对替硝唑敏感的H.pylori菌株中,vacA s1a,s1b和s2型分别占50.0%,40.0%和10.0%;vacA m1和m2型占40.0%和60.0%;vacA s1a/m1,s1b/m1,s1a/m2,s1b/m2和s2/m2型分别占20.0%,20.0%,30.0%,20.0%和10.0%;cagA+和cagA-型为80.0%和20.0%.结论 H pylori菌株对甲硝唑的耐药率明显高于H.pylori对克拉霉素,阿莫西林,替硝唑的耐药率,不同cagA vacA基因亚型的H.pylori菌株对替硝唑的耐药率无显著差异.
목적연구유문라간균(H.pylori)cagA vacA기인아형급기여항생소내약적관계.방법용경지희석법측정50례H.pylori림상분리주대극랍매소,아막서림,체초서,갑초서적최저억균농도(MIC),병계산MIC50화MIC90치.용다중PCR감정H.pylori균주적vacA(s1a,s1b,s2,m1,m2)급cagA적형별.결과극랍매소,아막서림,체초서,갑초서대H.pylori적MIC50화MIC90분별위:＜0.016 mg@L-1,0.016 mg@L-1;0.016mg@L-1,0.047 mg@L-1;16.000 mg@L-1,52.000 mg@L-1;25.140mg@L-1,76.800mg@L-1.H.pylori균주대극랍매소,아막서림,체초서,갑초서적내약솔분별위0%,0%,20%화38%.재대체초서민감적H.pylori균주중,vacA s1a,s1b화s2형분별점50.0%,42.5%화7.5%;vacA m1화m2형점60.0%화40.0%;vacA sla/m1,s1b/m1,s1a/m2,s1b/m2화s2/m2형분별점32.5%,27.5%,17.5%,15.0%화12.5%;cagA+화cagA-형위87.5%화12.5%.재대체초서민감적H.pylori균주중,vacA s1a,s1b화s2형분별점50.0%,40.0%화10.0%;vacA m1화m2형점40.0%화60.0%;vacA s1a/m1,s1b/m1,s1a/m2,s1b/m2화s2/m2형분별점20.0%,20.0%,30.0%,20.0%화10.0%;cagA+화cagA-형위80.0%화20.0%.결론 H pylori균주대갑초서적내약솔명현고우H.pylori대극랍매소,아막서림,체초서적내약솔,불동cagA vacA기인아형적H.pylori균주대체초서적내약솔무현저차이.