CAJ | 학술논문

目的研究腹腔注射(ip)3,4 -亚甲基二氧基甲基苯丙胺(MDMA)对实验大鼠大脑神经元的凋亡诱导作用,以及凋亡相关因子Caspase-3和细胞色表C(CytC)在不同脑区的表达情况.方法将20只大鼠均分为4组,随机选3组为MDMA实验组(B、C、D),另1组为对照组(A).B组予MDMA 20 mg/kg,ip,单次,C组予MDMA 20 mg/kg Bid×2 d,ip,D组予MDMA 20 mg/kg Bid×4 d,ip;A组给予等体积生理盐水(ip, 单次).采用TUNEL法检测神经元的凋亡,免疫组织化学方法检测Caspase-3和CytC的表达.结果与对照组相比,给予MDMA后,B、C和D组大鼠各相关脑区 (额叶皮层、海马和纹状体)有凋亡细胞形成,Caspase-3和CytC有程度不同的表达.C组和D组较B组的凋亡细胞增加且凋亡相关因子的表达增加(P<0.05).结论 MDMA可导致实验大鼠神经元的凋亡,并诱导凋亡相关因子Caspase-3和CytC的表达.
목적 연구복강주사(ip)3,4 -아갑기이양기갑기분병알(MDMA)대실험대서대뇌신경원적조망유도작용,이급조망상관인자Caspase-3화세포색표C(CytC)재불동뇌구적표체정황.방법 장20지대서균분위4조,수궤선3조위MDMA실험조(B、C、D),령1조위대조조(A).B조여MDMA 20 mg/kg,ip,단차,C조여MDMA 20 mg/kg Bid×2 d,ip,D조여MDMA 20 mg/kg Bid×4 d,ip;A조급여등체적생리염수(ip, 단차).채용TUNEL법검측신경원적조망,면역조직화학방법검측Caspase-3화CytC적표체.결과 여대조조상비,급여MDMA후,B、C화D조대서각상관뇌구 (액협피층、해마화문상체)유조망세포형성,Caspase-3화CytC유정도불동적표체.C조화D조교B조적조망세포증가차조망상관인자적표체증가(P<0.05).결론 MDMA가도치실험대서신경원적조망,병유도조망상관인자Caspase-3화CytC적표체.
Objective To study the neuron apoptosis induced by I. P 3.4-methylenedioxy methamphetamine (MDMA) and the expression of apoptosis-related factors in rat brain. Methods Twenty rats were divided into 4 groups. In group A, the rats were injected intraperitoneally with single dosage of saline, while the rats of group B, C, D were injected I. P with MDMA in different regimen, which were 20 mg/kg, single injection in group B, 20 mg/ kg twice a day (8 am and 8 pm) , for 2 day in group C, as well as 20 mg/kg, twice a day (8 am and 8 pm) for 4 days. Neuron apoptosis were measured by TUNEL, and the expression of Caspase-3 and CytC were detected by immunohistochemistry. Results Compared with saline group, apoptosis neurons were detected at the related brain regions (such as frontal cortex, hippocampus and striatum) of the rats in MDMA treated groups;Expression of Caspase-3 and CytC was observed at different level. Compared with group B, the number of apoptosis neurons of group C and D increased, and also the apoptosis-related factors in the brain tissue increased (P<0. 05). Conclusion MDMA could induce neurons apoptosis and the expression of apoptosis-related factors such as Caspase-3 and CytC in rat brain.Objective To study the neuron apoptosis induced by I. P 3.4-methylenedioxy methamphetamine (MDMA) and the expression of apoptosis-related factors in rat brain. Methods Twenty rats were divided into 4 groups. In group A, the rats were injected intraperitoneally with single dosage of saline, while the rats of group B, C, D were injected I. P with MDMA in different regimen, which were 20 mg/kg, single injection in group B, 20 mg/ kg twice a day (8 am and 8 pm) , for 2 day in group C, as well as 20 mg/kg, twice a day (8 am and 8 pm) for 4 days. Neuron apoptosis were measured by TUNEL, and the expression of Caspase-3 and CytC were detected by immunohistochemistry. Results Compared with saline group, apoptosis neurons were detected at the related brain regions (such as frontal cortex, hippocampus and striatum) of the rats in MDMA treated groups;Expression of Caspase-3 and CytC was observed at different level. Compared with group B, the number of apoptosis neurons of group C and D increased, and also the apoptosis-related factors in the brain tissue increased (P<0. 05). Conclusion MDMA could induce neurons apoptosis and the expression of apoptosis-related factors such as Caspase-3 and CytC in rat brain.