CAJ | 학술논문

目的: 观察中药抗纤灵方对转化生长因子β_1(TGF-β_1)刺激后肾小管上皮细胞(TECs)中肝细胞生长因子(HGF)受体c-Met及其下游丝裂原激活的蛋白激酶(MAPK)信号分子的调控作用,以初步探讨其延缓肾纤维化的作用机制.方法: 给予大鼠中药灌胃3 d后制备抗纤灵含药鼠血清,应用不同含药血清处理经TGF-β_1刺激的HK-2细胞,然后用Alarm blue法和Western blotting法检测细胞增殖率、c-Met和MAPK磷酸化情况.结果: TGF-β_1和含药鼠血清对HK-2细胞增殖率无明显影响,含药血清影响TGF-β_1刺激的HK-2细胞c-Met的表达及MAPK的磷酸化.结论: 抗纤灵含药血清可调节HGF受体c-Met介导的MAPK的磷酸化.
목적: 관찰중약항섬령방대전화생장인자β_1(TGF-β_1)자격후신소관상피세포(TECs)중간세포생장인자(HGF)수체c-Met급기하유사렬원격활적단백격매(MAPK)신호분자적조공작용,이초보탐토기연완신섬유화적작용궤제.방법: 급여대서중약관위3 d후제비항섬령함약서혈청,응용불동함약혈청처리경TGF-β_1자격적HK-2세포,연후용Alarm blue법화Western blotting법검측세포증식솔、c-Met화MAPK린산화정황.결과: TGF-β_1화함약서혈청대HK-2세포증식솔무명현영향,함약혈청영향TGF-β_1자격적HK-2세포c-Met적표체급MAPK적린산화.결론: 항섬령함약혈청가조절HGF수체c-Met개도적MAPK적린산화.
AIM: To observe the effects of sera containing Kang xian ling (a traditional Chinese medicine) on c-Met and its downstream MAPK signal molecules in HK-2 cells treated with transforming growth factor-β_1(TGF-β_1), so as to further explore the effective mechanism of anti-renal fibrosis of the traditional Chinese medicine. METHODS: Rat sera containing herbs were collected after rats were intragastrically administered with the herbs. HK-2 cells activated by TGF-β_1 were incubated with different heat-inactivated sera. The proliferation and expression of MAPK and the phosphorylation of MAPK in HK-2 cells were detected by Alarm blue and immunoblotting. RESULTS: No significant difference of cell proliferation between any two groups was observed. C-Met and the phosphorylation of its downstream molecules were also observed after incubated with different sera. CONCLUSION: The receptor c-Met of hepatocyte growth factor, and the phosphorylation of its downstream MAPK molecules can be regulated by the sera containing Kang xian ling.