CAJ | 학술논문

目的探讨免疫佐剂BCG-CpG-DNA对A群脑膜炎球菌多糖疫苗的作用.方法将不同剂量(5、10、100 μg)的免疫佐剂BCG-CpG-DNA与A群脑膜炎球菌多糖疫苗直接混合后,皮下免疫小鼠,与未添加佐剂疫苗比较,ELISA法检测抗原特异性IgG抗体水平,并分析抗体亚类IgG1和IgG2a的水平.结果 BCG-CpG-DNA能提高A群脑膜炎球菌多糖疫苗特异性抗体IgG水平,并能促进抗原特异性抗体亚类的产生,其中佐剂中、高剂量(10、100μg)实验组IgG1、IgG2a水平与未加入免疫佐剂疫苗之间差异有统计学意义.结论 BCG-CpG-DNA对A群脑膜炎球菌多糖疫苗有免疫佐剂作用.
목적 탐토면역좌제BCG-CpG-DNA대A군뇌막염구균다당역묘적작용.방법 장불동제량(5、10、100 μg)적면역좌제BCG-CpG-DNA여A군뇌막염구균다당역묘직접혼합후,피하면역소서,여미첨가좌제역묘비교,ELISA법검측항원특이성IgG항체수평,병분석항체아류IgG1화IgG2a적수평.결과 BCG-CpG-DNA능제고A군뇌막염구균다당역묘특이성항체IgG수평,병능촉진항원특이성항체아류적산생,기중좌제중、고제량(10、100μg)실험조IgG1、IgG2a수평여미가입면역좌제역묘지간차이유통계학의의.결론 BCG-CpG-DNA대A군뇌막염구균다당역묘유면역좌제작용.
Objective To study adjuvandty of BCG-CpO-DNA from Mycobactefium bovis strain BCG to group A meningococcal polysaccharide antigen.Methods Mice were vaccinated s.c.with group A meningococcal polysaccharide vaccine alone and combined with different doses of BCG-CpG-DNA (5,10,100 μg) ,then the humoral response were assessed.antigen-specifec IgG and antigen-specifec IgG isotypes were tested by ELISA and analysis of antibody subclasses IgG1 and IgG2a levels.Results BCG-CpG-DNA improved antibody level of anfigen-specifec IgG and IgG2a,the middle and high dose (10,100 μg) of BCG-CpG-DNA group are statistically significant (P<0.05) vs vaccine controll group respectfly.It moans that BCG-CpG-DNA induced Thl type immune responses.Conclusion BCG-CpG-DNA is a potential new immune adjuvant for group A meningococcal polysaccharide vaccine.