CAJ | 학술논문

背景:异体肝脏移植由主要组织相容性抗原可诱发免疫排斥反应,免疫抑制剂会对机体产生不良反应,目前通过移植前对供受体进行预处理等策略,可以诱导受体产生免疫耐受,在移植中具有广阔的应用前景.目的:用大鼠胸腺诱导的方法,对大鼠肝移植进行处理,从胸腺诱导方面,研究胸腺诱导与移植免疫排斥反应的关系.方法:供体为清洁级雄性SD大鼠40只,受体为清洁级雄性Wistar大鼠30只和清洁级雄性SD大鼠10只,分为同种基因移植组、同种异基因移植组、环孢素组、胸腺修饰诱导组,各10对.采用改良Kamada二袖套法并加以改进建立稳定的大鼠原位肝移植模型,造模后,环孢素组每天腹腔注射环孢索(50 mg/kg),持续5 d,胸腺修饰诱导组胸腺左右两叶各注射50 μL主要组织相容性抗原,持续5 d,其他组不给予任何干预措施,记录各组大鼠生存时间并分别于移植后3,7,14,21,28 d进行病理学观察和混合淋巴细胞培养.结果与结论:经胸腺修饰诱导的肝移植大鼠生存时间则显著延长(>60d),移植肝内组织损伤程度显著减轻,肝脏局部免疫排斥反应减少,淋巴细胞减少,肝移植效果与同种基因移植大鼠接近,且优于环孢素干预大鼠(P<0.05).提示胸腺诱导可减轻大鼠肝移植后产生的免疫排斥反应.
배경:이체간장이식유주요조직상용성항원가유발면역배척반응,면역억제제회대궤체산생불량반응,목전통과이식전대공수체진행예처리등책략,가이유도수체산생면역내수,재이식중구유엄활적응용전경.목적:용대서흉선유도적방법,대대서간이식진행처리,종흉선유도방면,연구흉선유도여이식면역배척반응적관계.방법:공체위청길급웅성SD대서40지,수체위청길급웅성Wistar대서30지화청길급웅성SD대서10지,분위동충기인이식조、동충이기인이식조、배포소조、흉선수식유도조,각10대.채용개량Kamada이수투법병가이개진건립은정적대서원위간이식모형,조모후,배포소조매천복강주사배포색(50 mg/kg),지속5 d,흉선수식유도조흉선좌우량협각주사50 μL주요조직상용성항원,지속5 d,기타조불급여임하간예조시,기록각조대서생존시간병분별우이식후3,7,14,21,28 d진행병이학관찰화혼합림파세포배양.결과여결론:경흉선수식유도적간이식대서생존시간칙현저연장(>60d),이식간내조직손상정도현저감경,간장국부면역배척반응감소,림파세포감소,간이식효과여동충기인이식대서접근,차우우배포소간예대서(P<0.05).제시흉선유도가감경대서간이식후산생적면역배척반응.
BACKGROUND: During xenogenic liver transplantation, major histocompatibility antigen can induce immunological rejection, and immunosuppressant can cause adverse effect on organism. Recently, treatment prior to transplantation induces immune tolerance, which is perspective for organ transplantation.OBJECTIVE: To investigate the correlation between thymus induction and immunological rejection during liver transplantation. METHODS: A total of 40 male SD rats of clean grade were selected as donors. Moreover, 30 male Wistar rats of clean grade and 10 male SD rats of clean grade were selected as recipients. The donor rats were divided into allogeneic gene transplantation, allotransplantation, cyclosporine, and thymus induction groups, with 10 rats in each group. The modified Kamada and improved two-cuff technique was used to establish a stable rat orthotopic liver transplantation model. The cyclosporine group was given cyclosporine (50 mg/kg) for 5 successive days. Thymus induction group was injected with major histocompatibility antigens (50 pL) for 5 successive days. Other groups were not given any interventions. Survival time of rats was recorded in each group. Pathological observation and mixed lymphocyte cultured were performed at days 3, 7, 14, 21, and 28 after transplantation. RESULTS AND CONCLUSION: Survival time was longer in the thymus induced group compared with other groups (> 60 days), damaged level was mild, local immunological rejection was reduced, and lymphocytes were decreased. The effect after liver transplantation was similar to allogeneic gene transplantation but superior to cyclosporine intervention (P < 0.05). This suggested that thymus induction relieved immunological rejection following liver transplantation.