CAJ | 학술논문

目的研究内源性一氧化氮(NO)对人乳腺癌细胞化疗敏感性的影响. 方法应用IL-1β处理培养的MCF-7细胞,检测NO的产生情况并用蛋白质印迹法检测诱导型一氧化氮合酶(in-duceble nitric oxide synthase,iNOS)蛋白的表达.M1rr法检测MCF-7细胞在NOS抑制剂NG-甲基-L-精氨酸(NG-monomethyl-L-arginine,L-NMMA)和NO合成原料L-精氨酸(L-arginine,L-Arg)作用下对多柔比星(adriamycin,ADM)和5-氟尿嘧啶(5-fluorouracil,5-Fu)药物的敏感性. 结果内源性NO的产量与IL-1β剂量呈正相关.在IL-1β诱导作用下MCF-7细胞大量表达iNOS蛋白,并与L-Arg、L-NMMA存在与否无关.当ADM浓度为0.5 μmol/L和1 μmol/L时,实验组细胞的生存率明显下降(P<0.05).加入L-NMMA能显著提高实验组细胞的生存率(P<0.05);加入L-Arg能显著提高MCF-7细胞对化疗药物的敏感性(P<0.05).结论在细胞因子IL-1β诱导下MCF-7细胞产生的内源性NO增加,并使MCF-7细胞化学敏感性提高.
목적 연구내원성일양화담(NO)대인유선암세포화료민감성적영향. 방법응용IL-1β처리배양적MCF-7세포,검측NO적산생정황병용단백질인적법검측유도형일양화담합매(in-duceble nitric oxide synthase,iNOS)단백적표체.M1rr법검측MCF-7세포재NOS억제제NG-갑기-L-정안산(NG-monomethyl-L-arginine,L-NMMA)화NO합성원료L-정안산(L-arginine,L-Arg)작용하대다유비성(adriamycin,ADM)화5-불뇨밀정(5-fluorouracil,5-Fu)약물적민감성. 결과내원성NO적산량여IL-1β제량정정상관.재IL-1β유도작용하MCF-7세포대량표체iNOS단백,병여L-Arg、L-NMMA존재여부무관.당ADM농도위0.5 μmol/L화1 μmol/L시,실험조세포적생존솔명현하강(P<0.05).가입L-NMMA능현저제고실험조세포적생존솔(P<0.05);가입L-Arg능현저제고MCF-7세포대화료약물적민감성(P<0.05).결론재세포인자IL-1β유도하MCF-7세포산생적내원성NO증가,병사MCF-7세포화학민감성제고.
Objective To evaluate the effects of endogenous NO on the chemosensitivity of human breast cancer cell. Methods MCF-7 cells were cultured as monolayer, incubated with cytokine IL-1β. The pro-duction of NO was detected by NO assay. The expression of iNOS protein was measured by Western blotting. Establishing control group and experimental group, the chemosensitivity of MCF-7 cells incubated by L-NMMA and L-Arg to ADM and 5-Fu was studied by MTT assay. Results There was a positive correlation of dose-dependence between NO production and IL-1β concentration. MCF-7 cells expressed plenty of iNOS by induetion of IL-1β. There was no significant difference on iNOS whether L-NMMA and L-Arg existed or not. Incubating MCF-7 cells with 0. 5 μmol/L or 1 μmol/L ADM, the survival rate of experiment group was remarkablely decreased(P < 0.05) ; L-NMMA significantly increased survival rate of experiment group(P < O. 05) ; L-Arg decreased survival rate of experiment group(P < 0.05). Conclusion The induction of IL-113 in MCF-7 cells can increase the production of endogenous NO, which increases MCF-7 cells' sensitivity to chemotherapy.