中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
1期
5-9
,共5页
权启萌%赵京龙%张贵祥%顾青
權啟萌%趙京龍%張貴祥%顧青
권계맹%조경룡%장귀상%고청
超顺磁性氧化铁%多聚左旋赖氨酸%磁共振成像%C6脑胶质瘤%肿瘤标记,生物学
超順磁性氧化鐵%多聚左鏇賴氨痠%磁共振成像%C6腦膠質瘤%腫瘤標記,生物學
초순자성양화철%다취좌선뢰안산%자공진성상%C6뇌효질류%종류표기,생물학
Super paramagnetic iron oxide%Poly-L-lysine%Magnetic resonance imaging%C6 Glioma%Tumor markers,biological
目的 观察多聚左旋赖氨酸(PLL)协同超顺磁性氧化铁(SPIO)对大鼠C6胶质瘤细胞的标记率及对标记细胞生物活性的影响.方法 将未标记的C6胶质瘤细胞设为对照组,25 mg/L SPIO(A组)、25mg,LSPIO+0.75mg,LPLL(B组)、50mg,LSPIO+1.5mg,LPLL(C组)标记的C6胶质瘤细胞设为实验组.各组细胞处理后分别培养6、24及48 h进行MTS细胞活性检测;普鲁士蓝染色评定标记率;3.0 T MRI GRE/30° T2*WI序列对各组细胞体外成像,比较R2*值及信号强度差异.结果 各实验组细胞处理后48 h均未见细胞活性显著降低(均P>0.05).普鲁士蓝染色显示B组、C组细胞标记率均达到98%以上,而A组约为70%,SPIO与PLL混合标记细胞随SPIO浓度增高染色程度逐渐加深.3.0T MRI体外细胞成像对标记细胞有较敏感的信号变化,对照组及A、B、C组R2*值分别为11.76±5.74、12.13±4.39、61.22±27.85、90.07±35.59,对照组与A组间R2*值差异无统计学意义(P>0.05);B、C组分别与其它各组间比较差异均有统计学意义,且此两组之间差异亦有统计学意义(均P<0.01).结论 PLL可增强SPIO对C6脑胶质瘤细胞的标记作用并对标记细胞活性未产生明显影响.3.0T MRI GRE/30°T2*WI序列对离体标记细胞有较敏感的信号变化,R2*值随着细胞内SPIO浓度的增加而增大.25 mg/L SPIO+0.75 mg,L PLL可获得满意的体外标记效果.
目的 觀察多聚左鏇賴氨痠(PLL)協同超順磁性氧化鐵(SPIO)對大鼠C6膠質瘤細胞的標記率及對標記細胞生物活性的影響.方法 將未標記的C6膠質瘤細胞設為對照組,25 mg/L SPIO(A組)、25mg,LSPIO+0.75mg,LPLL(B組)、50mg,LSPIO+1.5mg,LPLL(C組)標記的C6膠質瘤細胞設為實驗組.各組細胞處理後分彆培養6、24及48 h進行MTS細胞活性檢測;普魯士藍染色評定標記率;3.0 T MRI GRE/30° T2*WI序列對各組細胞體外成像,比較R2*值及信號彊度差異.結果 各實驗組細胞處理後48 h均未見細胞活性顯著降低(均P>0.05).普魯士藍染色顯示B組、C組細胞標記率均達到98%以上,而A組約為70%,SPIO與PLL混閤標記細胞隨SPIO濃度增高染色程度逐漸加深.3.0T MRI體外細胞成像對標記細胞有較敏感的信號變化,對照組及A、B、C組R2*值分彆為11.76±5.74、12.13±4.39、61.22±27.85、90.07±35.59,對照組與A組間R2*值差異無統計學意義(P>0.05);B、C組分彆與其它各組間比較差異均有統計學意義,且此兩組之間差異亦有統計學意義(均P<0.01).結論 PLL可增彊SPIO對C6腦膠質瘤細胞的標記作用併對標記細胞活性未產生明顯影響.3.0T MRI GRE/30°T2*WI序列對離體標記細胞有較敏感的信號變化,R2*值隨著細胞內SPIO濃度的增加而增大.25 mg/L SPIO+0.75 mg,L PLL可穫得滿意的體外標記效果.
목적 관찰다취좌선뢰안산(PLL)협동초순자성양화철(SPIO)대대서C6효질류세포적표기솔급대표기세포생물활성적영향.방법 장미표기적C6효질류세포설위대조조,25 mg/L SPIO(A조)、25mg,LSPIO+0.75mg,LPLL(B조)、50mg,LSPIO+1.5mg,LPLL(C조)표기적C6효질류세포설위실험조.각조세포처리후분별배양6、24급48 h진행MTS세포활성검측;보로사람염색평정표기솔;3.0 T MRI GRE/30° T2*WI서렬대각조세포체외성상,비교R2*치급신호강도차이.결과 각실험조세포처리후48 h균미견세포활성현저강저(균P>0.05).보로사람염색현시B조、C조세포표기솔균체도98%이상,이A조약위70%,SPIO여PLL혼합표기세포수SPIO농도증고염색정도축점가심.3.0T MRI체외세포성상대표기세포유교민감적신호변화,대조조급A、B、C조R2*치분별위11.76±5.74、12.13±4.39、61.22±27.85、90.07±35.59,대조조여A조간R2*치차이무통계학의의(P>0.05);B、C조분별여기타각조간비교차이균유통계학의의,차차량조지간차이역유통계학의의(균P<0.01).결론 PLL가증강SPIO대C6뇌효질류세포적표기작용병대표기세포활성미산생명현영향.3.0T MRI GRE/30°T2*WI서렬대리체표기세포유교민감적신호변화,R2*치수착세포내SPIO농도적증가이증대.25 mg/L SPIO+0.75 mg,L PLL가획득만의적체외표기효과.
Objective To investigate the labeling rate with poly-L-lysine (PLL) and super paramagnetic iron oxide particles (SPIO) for C6 rat glioma cells and the impact of PLL and SPIO on labeled cell bioactivity. Methods C6 rat glioma cells were incubated without SPIO or PLL (control group), with 25 mg/L SPIO (group A), 25 mg/L SPIO+0.75 mg/L PLL (group B), and 50 mg/L SPIO+1.5 mg/L PLL (group C) , respectively. The labeled cell bioactivity was analyzed by MTS assay (mono-nuclear cell direct cvtotoxicity assay) at different incubation time (6, 24 and 48 h) after treatment, and the labeling rate was detected by Prussian Blue staining. The labeled cells mass were imaged in vitro using a 3.0 T MRI scanner with GRE/30° T2*WI sequence. The R2* values and signal intensity were compared among these groups. Results Up to 48 h post-labeling, there was no significant decrease of cell bioactivity in all labeled cell groups (all P>0.05). Prussian Blue staining showed that the labeling rate was >98% in group B and C compared with about 70% in group A. The staining appeared increasingly darker in SPIO/PLL mixed labeled cells along with higher concentration of SPIO. 3.0 T MRI was signal-sensitive for the labeled cells. The R2* values were 11.76±5.74, 12.13±4.39, 61.22±27.85 and 90.07±35.59 for control group and group A, B and C, respectively. Difference of R2* value was not found between control group and group A (P>0.05), but was found between group B and C, meanwhile R* values of group B and C were significantly different with control group and group A (all P<0.01). Conclusions PLL may enhance the efficiency of SPIO labeling for C6 glioma cells and has no significant impact on the cell bioactivity. 3.0 T MRI with GRE/30° T2*WI sequence is signal-sensitive for labeled cells in vitro. The R2* value may increase along with intracellular SPIO level. 25 mg/L SPIO+0.75 mg/L PLL may yield satisfactory labeling for rat C6 glioma cells in vitro.
