中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
2期
97-102
,共6页
狄氏剂%纹状体%突触发育
狄氏劑%紋狀體%突觸髮育
적씨제%문상체%돌촉발육
Dieldrin%Striatum%Synaptic development
目的 探讨出生后早期接触狄氏剂后对ICR小鼠哺乳期、青春期和成年期纹状体突触发育的影响.方法 出生后每窝小鼠数量经调整后,随机分为5组,分别为0.2、2.0和20.0 μg/kg 3个狄氏剂染毒组及溶剂对照组和生理盐水组,于出生后第3天(PND3)进行腹腔注射染毒,隔天1次,连续6次,分别在哺乳期(PND14)、青春期(PND36)和成年期(PND98)分离纹状体.采用免疫印迹法检测纹状体相关突触蛋白水平.结果 出生后早期狄氏剂染毒,在哺乳期可降低小鼠纹状体中生长相关蛋白( GAP43)水平,且存在剂量依赖性；至青春期,纹状体中胶质原纤维酸性蛋白(GFAP)水平升高,酪氨酸羟化酶(TH)、GAP43和突触后致密蛋白(PSD95)水平均较溶剂对照组下降,存在剂量-效应关系,同时在雄性小鼠中还出现突触蛋白Ⅰ (Synapsin Ⅰ)水平下降；GFAP、TH和PSD95的这种改变一直持续到成年以后.结论 出生后脑发育关键期接触狄氏剂可影响小鼠纹状体中GAP43水平,在此后的生命阶段(包括青春期和成年期),小鼠纹状体中多巴胺能神经元特异性蛋白(TH)和PSD95水平均下降,说明出生后早期短期接触低剂量狄氏剂会持久性干扰纹状体的突触发育.
目的 探討齣生後早期接觸狄氏劑後對ICR小鼠哺乳期、青春期和成年期紋狀體突觸髮育的影響.方法 齣生後每窩小鼠數量經調整後,隨機分為5組,分彆為0.2、2.0和20.0 μg/kg 3箇狄氏劑染毒組及溶劑對照組和生理鹽水組,于齣生後第3天(PND3)進行腹腔註射染毒,隔天1次,連續6次,分彆在哺乳期(PND14)、青春期(PND36)和成年期(PND98)分離紋狀體.採用免疫印跡法檢測紋狀體相關突觸蛋白水平.結果 齣生後早期狄氏劑染毒,在哺乳期可降低小鼠紋狀體中生長相關蛋白( GAP43)水平,且存在劑量依賴性；至青春期,紋狀體中膠質原纖維痠性蛋白(GFAP)水平升高,酪氨痠羥化酶(TH)、GAP43和突觸後緻密蛋白(PSD95)水平均較溶劑對照組下降,存在劑量-效應關繫,同時在雄性小鼠中還齣現突觸蛋白Ⅰ (Synapsin Ⅰ)水平下降；GFAP、TH和PSD95的這種改變一直持續到成年以後.結論 齣生後腦髮育關鍵期接觸狄氏劑可影響小鼠紋狀體中GAP43水平,在此後的生命階段(包括青春期和成年期),小鼠紋狀體中多巴胺能神經元特異性蛋白(TH)和PSD95水平均下降,說明齣生後早期短期接觸低劑量狄氏劑會持久性榦擾紋狀體的突觸髮育.
목적 탐토출생후조기접촉적씨제후대ICR소서포유기、청춘기화성년기문상체돌촉발육적영향.방법 출생후매와소서수량경조정후,수궤분위5조,분별위0.2、2.0화20.0 μg/kg 3개적씨제염독조급용제대조조화생리염수조,우출생후제3천(PND3)진행복강주사염독,격천1차,련속6차,분별재포유기(PND14)、청춘기(PND36)화성년기(PND98)분리문상체.채용면역인적법검측문상체상관돌촉단백수평.결과 출생후조기적씨제염독,재포유기가강저소서문상체중생장상관단백( GAP43)수평,차존재제량의뢰성；지청춘기,문상체중효질원섬유산성단백(GFAP)수평승고,락안산간화매(TH)、GAP43화돌촉후치밀단백(PSD95)수평균교용제대조조하강,존재제량-효응관계,동시재웅성소서중환출현돌촉단백Ⅰ (Synapsin Ⅰ)수평하강；GFAP、TH화PSD95적저충개변일직지속도성년이후.결론 출생후뇌발육관건기접촉적씨제가영향소서문상체중GAP43수평,재차후적생명계단(포괄청춘기화성년기),소서문상체중다파알능신경원특이성단백(TH)화PSD95수평균하강,설명출생후조기단기접촉저제량적씨제회지구성간우문상체적돌촉발육.
Objective To investigate the effects of early postnatal exposure to dieldrin on striatum synaptic development in lactation,adolescence and adulthood of mice.Methods The pups were divided into 5 groups randomly.Three groups were exposed to dieldrin (0.01％ DMSO solution) at doses of 0.2,2.0 and 20.0 μg/kg and two control groups were exposed to DMSO or saline by intraperitoneal injection of every other day from postnatal days (PND) 3 to PND13.The striatum were isolated from brain in lactation (PND14),adolescence (PND36) and adulthood (PND98).Western blot assay was used to detect the expression levels of striatal synaptic proteins.Results The postnatal exposure to dieldrin could reduce the level of growth associated protein (GAP43) of striatum in lactation in a dose-dependent manner.In adolescence,the level of glial fibrillary acidic protein (GFAP) in striatum increased and the levels of tyrosine hydroxylase (TH),GAP43 and post-synaptic density protein 95 (PSD95) decreased with exposure doses.The level of Synapsin Ⅰ decreased in adolescence male mice.The changes of expression levels of GFAP,TH and PSD95 proteins lasted to adulthood.Conclusion Early postnatal exposure to dieldrin could affect the expression level of GAP43 protein in striatum.The expression levels of TH and PSD95 proteins in striatum decreased in adolescence and adulthood.These results indicated that the early postnatal exposure to dieldrin may persistently interfere in the striatal synaptic development.
