CAJ | 학술논문

以Kinmaze(粳稻)/DV85(籼稻)的重组自交系F10世代群体检测了影响水稻低温发芽力性状的数量性状基因座(QTL).通过测定不同时期的低温发芽率,确定了15℃低温、第10 d为检测低温发芽率的最适处理温度和时间,该条件下能够充分检测到品种的差异和分离群体的变异.通过设置对照,证明所检测的低温发芽率不受休眠及二次休眠的影响.15℃低温、第10 d时,Kinmaze的发芽率达35%,DV85的发芽率只有7%,两亲本之间存在明显差异,该群体81个家系的低温发芽率变幅在0%～99%之间.QTL分析结果检测到5个与低温发芽力相关的基因座,分别位于第2、6、7、11和12染色体上.位于第2、6和11染色体上的qLTG-2、 qLTG-6和qLTG-11贡献率分别为27.1%、17.1%和15.0%,对低温发芽力性状的增效基因来自DV85;位于第7、12染色体上qLTG-7和qLTG-12的贡献率分别为22.9%和8.8%,增效基因来自Kinmaze.其中,qLTG-6和qLTG-11在染色体上的位置与已报道的有关低温发芽力QTL位置相似,而qLTG-2、qLTG-7和qLTG-12为新检测的低温发芽力基因座.上位性分析结果显示,第3与第5染色体上存在影响低温发芽力的互作位点,其互作可以提高低温发芽力,而第7染色体上的两位点之间的互作降低了低温发芽力.
이Kinmaze(갱도)/DV85(선도)적중조자교계F10세대군체검측료영향수도저온발아력성상적수량성상기인좌(QTL).통과측정불동시기적저온발아솔,학정료15℃저온、제10 d위검측저온발아솔적최괄처리온도화시간,해조건하능구충분검측도품충적차이화분리군체적변이.통과설치대조,증명소검측적저온발아솔불수휴면급이차휴면적영향.15℃저온、제10 d시,Kinmaze적발아솔체35%,DV85적발아솔지유7%,량친본지간존재명현차이,해군체81개가계적저온발아솔변폭재0%～99%지간.QTL분석결과검측도5개여저온발아력상관적기인좌,분별위우제2、6、7、11화12염색체상.위우제2、6화11염색체상적qLTG-2、 qLTG-6화qLTG-11공헌솔분별위27.1%、17.1%화15.0%,대저온발아력성상적증효기인래자DV85;위우제7、12염색체상qLTG-7화qLTG-12적공헌솔분별위22.9%화8.8%,증효기인래자Kinmaze.기중,qLTG-6화qLTG-11재염색체상적위치여이보도적유관저온발아력QTL위치상사,이qLTG-2、qLTG-7화qLTG-12위신검측적저온발아력기인좌.상위성분석결과현시,제3여제5염색체상존재영향저온발아력적호작위점,기호작가이제고저온발아력,이제7염색체상적량위점지간적호작강저료저온발아력.
Quantitative trait loci (QTL) controlling low temperature germinability (LTG) in rice were identified using 81 recombinant inbreed lines (RILs) derived from a cross between a japonica variety Kinmaze and an indica variety DV85.The accurate condition of LTG evaluation was assumed at 15℃ for 10 d after removing the effect of dormancy and the second dormancy.By setting check,the scores of LTG in this study were evaluated.The germination rate at 15℃ for 10 d was scored to represent the LTG.The LTG of the RILs ranged from 0 to 99%.By single point analysis,simple interval mapping,and composite interval mapping,5 putative QTL,qLTG-2,qLTG-6,qLTG-7,qLTG-11 and qLTG-12 were detected on chromosomes 2,6,7,11 and 12 respectively.At the regions of qLTG-2,qLTG-6 and qLTG-11,DV85 alleles increased the LTG,while Kinmaze alleles increased it at the regions of qLTG-7 and qLTG-12.Among the five QTLs reported here,qLTG-2,qLTG-7 and qLTG-12 were newly detected,while the other two QTL-containing regions were close to those previously reported.Epistatic QTL were also detected in this paper.