肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2010年
4期
243-246
,共4页
肿瘤%实验性%卵巢肿瘤%苏木水提物%细胞凋亡
腫瘤%實驗性%卵巢腫瘤%囌木水提物%細胞凋亡
종류%실험성%란소종류%소목수제물%세포조망
Neoplasms,experimental%Ovarian neoplasms%Caesalpinia sappan aqueous extract's%Apoptosis
目的 观察苏木水提物对人类浆液性囊腺卵巢癌细胞SKOV3的生长抑制及诱导凋亡作用.方法 采用细胞计数法绘制生长抑制曲线;光镜下观察药物作用后SKOV3细胞经锥虫蓝染色后的形态变化;电子显微镜下观察苏木水提物作用后细胞的超微结构;不同质量浓度苏木水提物作用于细胞后,用流式细胞术(FCM)进行凋亡及周期分析.结果 生长曲线显示苏木水提物对SKOV3细胞有明显的生长抑制作用,且呈现一定的质量浓度依赖性;电镜图可见典型的细胞凋亡特征,如细胞核固缩、染色质凝聚及凋亡小体形成;FCM分析证实:体外培养的SKOV3细胞经不同质量浓度的苏木水提物作用后,细胞生长明显受到抑制,细胞凋亡率随苏木水提物质量浓度的增加而增加.结论 苏木水提物对人类卵巢癌细胞株SKOV3有明显的生长抑制及诱导凋亡作用.
目的 觀察囌木水提物對人類漿液性囊腺卵巢癌細胞SKOV3的生長抑製及誘導凋亡作用.方法 採用細胞計數法繪製生長抑製麯線;光鏡下觀察藥物作用後SKOV3細胞經錐蟲藍染色後的形態變化;電子顯微鏡下觀察囌木水提物作用後細胞的超微結構;不同質量濃度囌木水提物作用于細胞後,用流式細胞術(FCM)進行凋亡及週期分析.結果 生長麯線顯示囌木水提物對SKOV3細胞有明顯的生長抑製作用,且呈現一定的質量濃度依賴性;電鏡圖可見典型的細胞凋亡特徵,如細胞覈固縮、染色質凝聚及凋亡小體形成;FCM分析證實:體外培養的SKOV3細胞經不同質量濃度的囌木水提物作用後,細胞生長明顯受到抑製,細胞凋亡率隨囌木水提物質量濃度的增加而增加.結論 囌木水提物對人類卵巢癌細胞株SKOV3有明顯的生長抑製及誘導凋亡作用.
목적 관찰소목수제물대인류장액성낭선란소암세포SKOV3적생장억제급유도조망작용.방법 채용세포계수법회제생장억제곡선;광경하관찰약물작용후SKOV3세포경추충람염색후적형태변화;전자현미경하관찰소목수제물작용후세포적초미결구;불동질량농도소목수제물작용우세포후,용류식세포술(FCM)진행조망급주기분석.결과 생장곡선현시소목수제물대SKOV3세포유명현적생장억제작용,차정현일정적질량농도의뢰성;전경도가견전형적세포조망특정,여세포핵고축、염색질응취급조망소체형성;FCM분석증실:체외배양적SKOV3세포경불동질량농도적소목수제물작용후,세포생장명현수도억제,세포조망솔수소목수제물질량농도적증가이증가.결론 소목수제물대인류란소암세포주SKOV3유명현적생장억제급유도조망작용.
Objective To observe caesalpinia sappan aqueous extract's growth inhibiting and apoptosis inducing effects on human ovarian cancer cell line SKOV3. Methods Cell growth inhibiting effect was detected by cytometry; Examined were by trypan blue staining under a light microscope; Electronic microscopy was used to observe ultrastructure of SKOV3 cell affected by caesalpinia sappan aqueous extract's;With different concentration of caesalpinia sappan aqueous extract's acting on cells, cells cycle and apoptosis rate were analyzed by flow cytometry (FCM). Results The growth of SKOV3 was inhibited effectively by caesalpinia sappan aqueous extract's; typical morphology of apoptosis (cell shrinkage, chromatin condensation and apoptotic body formation) were observed and in a concentration-dependent manner. Flow cytometry detection showed: the experiment in vitro revealed growth of SKOV3 after treatment by caesalpinia sappan aqueous extract's at the different concentration. Apoptosis rate was increased with the increasing of the concentration of caesalpinia sappan aqueous extract's. Conclusion Caesalpinia sappan aqueous extract's can obviously inhibit growth of human ovarian cancer cell line SKOV3 and induce its apoptosis.
