中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2012年
7期
591-597
,共7页
宗元娟%沈强%张学东%雷博%方晏红%陈雪梅
宗元娟%瀋彊%張學東%雷博%方晏紅%陳雪梅
종원연%침강%장학동%뢰박%방안홍%진설매
糖尿病视网膜病变%疾病模型,动物%环孢素A%视网膜电图%超微结构
糖尿病視網膜病變%疾病模型,動物%環孢素A%視網膜電圖%超微結構
당뇨병시망막병변%질병모형,동물%배포소A%시망막전도%초미결구
Diabetic retinopathy%Disease model,animal%Cyclosporin A%Electroretinogram%Ultrastructure
目的 探讨玻璃体腔注射环孢素A(CsA)对早期糖尿病(DM)大鼠视网膜功能、组织结构及超微结构的影响.方法 实验研究.SD大鼠经链脲佐菌素诱导建立DM动物模型.实验分为正常对照组、未注射药物组(DM组)、注射CsA干预组(DM+ CsA组)、注射对照液干预组(DM+DMSO组),每组9只.于建模成功后4周进行玻璃体腔注射,注射后48 h应用视网膜电图(ERG)检测视网膜功能.然后处死大鼠获取视网膜,应用光镜、透射电镜检测视网膜结构变化.运用单因素方差分析方法分析4组大鼠在不同刺激光强下ERG结果的差异,辅以事后检验进行ERG结果的各组间多重比较.结果 不同刺激光强度下4组大鼠的ERG b波振幅间的差异有统计学意义(F=14.760～28.890,P值均＜0.01)；当刺激光强度≥-0.35 log cd · s·m-2时,4组大鼠的暗适应ERG a波振幅间的差异亦有统计学意义(F值分别为12.510和15.500,P值均＜0.01).与正常对照组(ERG a波:82.43±26.68,ERG b波:208.40±51.20)相比,DM组(ERG a波:39.71±7.61,ERG b波:92.20±24.42)和DM+ DMSO组(ERG a波:37.63±17.25,ERG b波:93.11±22.50)大鼠暗适应时均有ERG a波、b波振幅下降(t=5.448～7.872,P值均＜0.05),DM+ CsA组b波振幅(160.10±43.49)下降明显(t=3.299,P ＜0.05),但a波(63.91 ±20.32)无明显变化(P＞0.05).石蜡切片染色结果显示,与正常对照组相比,其他三组大鼠视网膜外核层疏松变薄、细胞超微结构紊乱呈凋亡表现.与DM组及DM+ DMSO组相比,DM+ CsA组暗适应时ERG a波、b波振幅下降程度轻(t=3.203-4.759,P值均＜0.05)、外核层疏松变薄程度轻、超微结构改变程度轻.结论 DM大鼠病程早期即出现视网膜神经功能的损害,并有视网膜神经细胞超微结构改变.眼内注射CsA对大鼠视网膜功能有保护作用.
目的 探討玻璃體腔註射環孢素A(CsA)對早期糖尿病(DM)大鼠視網膜功能、組織結構及超微結構的影響.方法 實驗研究.SD大鼠經鏈脲佐菌素誘導建立DM動物模型.實驗分為正常對照組、未註射藥物組(DM組)、註射CsA榦預組(DM+ CsA組)、註射對照液榦預組(DM+DMSO組),每組9隻.于建模成功後4週進行玻璃體腔註射,註射後48 h應用視網膜電圖(ERG)檢測視網膜功能.然後處死大鼠穫取視網膜,應用光鏡、透射電鏡檢測視網膜結構變化.運用單因素方差分析方法分析4組大鼠在不同刺激光彊下ERG結果的差異,輔以事後檢驗進行ERG結果的各組間多重比較.結果 不同刺激光彊度下4組大鼠的ERG b波振幅間的差異有統計學意義(F=14.760～28.890,P值均＜0.01)；噹刺激光彊度≥-0.35 log cd · s·m-2時,4組大鼠的暗適應ERG a波振幅間的差異亦有統計學意義(F值分彆為12.510和15.500,P值均＜0.01).與正常對照組(ERG a波:82.43±26.68,ERG b波:208.40±51.20)相比,DM組(ERG a波:39.71±7.61,ERG b波:92.20±24.42)和DM+ DMSO組(ERG a波:37.63±17.25,ERG b波:93.11±22.50)大鼠暗適應時均有ERG a波、b波振幅下降(t=5.448～7.872,P值均＜0.05),DM+ CsA組b波振幅(160.10±43.49)下降明顯(t=3.299,P ＜0.05),但a波(63.91 ±20.32)無明顯變化(P＞0.05).石蠟切片染色結果顯示,與正常對照組相比,其他三組大鼠視網膜外覈層疏鬆變薄、細胞超微結構紊亂呈凋亡錶現.與DM組及DM+ DMSO組相比,DM+ CsA組暗適應時ERG a波、b波振幅下降程度輕(t=3.203-4.759,P值均＜0.05)、外覈層疏鬆變薄程度輕、超微結構改變程度輕.結論 DM大鼠病程早期即齣現視網膜神經功能的損害,併有視網膜神經細胞超微結構改變.眼內註射CsA對大鼠視網膜功能有保護作用.
목적 탐토파리체강주사배포소A(CsA)대조기당뇨병(DM)대서시망막공능、조직결구급초미결구적영향.방법 실험연구.SD대서경련뇨좌균소유도건립DM동물모형.실험분위정상대조조、미주사약물조(DM조)、주사CsA간예조(DM+ CsA조)、주사대조액간예조(DM+DMSO조),매조9지.우건모성공후4주진행파리체강주사,주사후48 h응용시망막전도(ERG)검측시망막공능.연후처사대서획취시망막,응용광경、투사전경검측시망막결구변화.운용단인소방차분석방법분석4조대서재불동자격광강하ERG결과적차이,보이사후검험진행ERG결과적각조간다중비교.결과 불동자격광강도하4조대서적ERG b파진폭간적차이유통계학의의(F=14.760～28.890,P치균＜0.01)；당자격광강도≥-0.35 log cd · s·m-2시,4조대서적암괄응ERG a파진폭간적차이역유통계학의의(F치분별위12.510화15.500,P치균＜0.01).여정상대조조(ERG a파:82.43±26.68,ERG b파:208.40±51.20)상비,DM조(ERG a파:39.71±7.61,ERG b파:92.20±24.42)화DM+ DMSO조(ERG a파:37.63±17.25,ERG b파:93.11±22.50)대서암괄응시균유ERG a파、b파진폭하강(t=5.448～7.872,P치균＜0.05),DM+ CsA조b파진폭(160.10±43.49)하강명현(t=3.299,P ＜0.05),단a파(63.91 ±20.32)무명현변화(P＞0.05).석사절편염색결과현시,여정상대조조상비,기타삼조대서시망막외핵층소송변박、세포초미결구문란정조망표현.여DM조급DM+ DMSO조상비,DM+ CsA조암괄응시ERG a파、b파진폭하강정도경(t=3.203-4.759,P치균＜0.05)、외핵층소송변박정도경、초미결구개변정도경.결론 DM대서병정조기즉출현시망막신경공능적손해,병유시망막신경세포초미결구개변.안내주사CsA대대서시망막공능유보호작용.
Objective To investigate the electrophysiological and morphological changes of retinae after intravitreal injection of cyclosporin A ( CsA ) in experimental diabetic rats.Methods Experimental study.SD diabetic mellitus ( DM ) rat models were induced with intraperitoneal injection of streptozotocin.The rats were divided into four groups,with 9 rats in each group:the normal control ( group CON ),the diabetic rats with or without CsA intravitreal injection respectively( group DM + CsA and group DM ),and the diabetic rats with dimethyl sulfoxide ( DMSO ) intravitreal injection ( group DM + DMSO ).The intravitreal injection of CsA or contrast solution was performed 4 weeks after the modeling.The retinal function of rats was examined by electroretinogram (ERG) 48 hours after the intravitreal injection.All rats were sacrificed and the structural changes of retina were observed by optical and transmission electron microscope.The datas of ERG including amplitudes and latencies of a-wave and b-wave were analyzed by One-way ANOVA and Bonferroni post hoc test.Results There was difference in ERG b-wave among the 4 groups under different stimuli light intensity ( F =14.760-28.890,all P value ＜ 0.01 ).And there was difference in ERG a-wave among the 4 groups when the stimuli light intensity ≥-0.35 log cd · s · m-2 ( F =12.510,15.500,both P value ＜ 0.01 ).Comparing to the group CON ( ERG a-wave:82.43 ± 26.68,ERG b-wave:208.40± 51.20 ),the ERG a-wave and b-wave amplitudes of group DM ( ERG a-wave:39.71 ± 7.61,ERG b-wave:92.20 ±24.42) and group DM + DMSO ( ERG a-wave:37.63 ± 17.25,ERG b-wave:93.11 ± 22.50 ) ( t =5.448-7.872,all P value ＜ 0.05 ),and the ERG b-wave of group DM + CsA ( 160.10 ± 43.39 ) ( t =3.299,P ＜0.05 ) were declined in dark adaptation,while the ERG a-wave (63.91 ± 20.32) of group DM ± CsA had no difference ( P ＞ 0.05 ).The a-wave and b-wave amplitudes of group DM + CsA were significantly increased compared to the other two groups in dark adaptation ( t =3.203-4.759,both P ＜ 0.05 ).Under optical microscope,group DM,DM + DMSO and DM + CsA had outer nuclear layer ( ONL ) disorder.Under transmission electron microscope,there were uhrastructure changes of the three groups.Furthermore,the ONL disorder and uhrastructure changes of group DM + CsA were less severe than the other two groups.Conclusions Functional and morphological changes are evident in the early stages in DM rats.Intravitreal injection with CsA shows protective effects on the nerve function in DM retina.
