中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2010年
3期
166-168
,共3页
谭来勋%叶心国%刘志超%孙圣刚
譚來勛%葉心國%劉誌超%孫聖剛
담래훈%협심국%류지초%손골강
突触素%生长相关蛋白%运动功能%脑梗死
突觸素%生長相關蛋白%運動功能%腦梗死
돌촉소%생장상관단백%운동공능%뇌경사
Synaptophysin%Growth associated protein-43%Motor skill%Cerebral infarction
目的 研究运动训练对大鼠梗死灶周突触素mRNA和生长相关蛋白(GAP-43)mRNA的表达水平和大鼠运动功能的影响.方法 将肾性高血压后的大脑中动脉闭塞脑梗死模型大鼠100只分成训练组和对照组(n=50),训练组大鼠进行运动训练,对照组大鼠常规饲养.2组大鼠均于制模成功后第3,7,14天采用横木行走实验评定各亚组大鼠运动功能,在制模成功后第1,3,7,14,28天时用半定量逆转录-多聚酶链式反应法检测各组大鼠脑梗死灶周边区突触素mRNA、GAP-43 mRNA水平.结果 造模后第7,14天,2组大鼠运动功能得分与本组造模后第3天比较,差异有统计学意义(P<0.01);制模后第7,14天时,训练组运动功能得分[分别为(3.2±0.3)分和(5.8±0.9)分]显著高于对照组[分别为(1.6±0.4)分和(2.6±0.8)分],差异有统计学意义(P<0.01).2组大鼠脑梗死灶周突触素mRNA和GAP-43 mRNA水平均在造模后第1,3,7天时逐渐增高,差异有统计学意义(P<0.01).训练组突触素mRNA水平在造模后第3,7,14和28天时均高于对照组,差异有统计学意义(P<0.01);训练组GAP-43 mRNA水平仅在造模后第3和第7天时(0.295±0.03、0.512±0.045)高于对照组,差异有统计学意义(P<0.01).结论 运动训练可使大鼠脑梗死灶周突触素mRNA和GAP-43 mRNA水平增高,促进运动功能恢复.
目的 研究運動訓練對大鼠梗死竈週突觸素mRNA和生長相關蛋白(GAP-43)mRNA的錶達水平和大鼠運動功能的影響.方法 將腎性高血壓後的大腦中動脈閉塞腦梗死模型大鼠100隻分成訓練組和對照組(n=50),訓練組大鼠進行運動訓練,對照組大鼠常規飼養.2組大鼠均于製模成功後第3,7,14天採用橫木行走實驗評定各亞組大鼠運動功能,在製模成功後第1,3,7,14,28天時用半定量逆轉錄-多聚酶鏈式反應法檢測各組大鼠腦梗死竈週邊區突觸素mRNA、GAP-43 mRNA水平.結果 造模後第7,14天,2組大鼠運動功能得分與本組造模後第3天比較,差異有統計學意義(P<0.01);製模後第7,14天時,訓練組運動功能得分[分彆為(3.2±0.3)分和(5.8±0.9)分]顯著高于對照組[分彆為(1.6±0.4)分和(2.6±0.8)分],差異有統計學意義(P<0.01).2組大鼠腦梗死竈週突觸素mRNA和GAP-43 mRNA水平均在造模後第1,3,7天時逐漸增高,差異有統計學意義(P<0.01).訓練組突觸素mRNA水平在造模後第3,7,14和28天時均高于對照組,差異有統計學意義(P<0.01);訓練組GAP-43 mRNA水平僅在造模後第3和第7天時(0.295±0.03、0.512±0.045)高于對照組,差異有統計學意義(P<0.01).結論 運動訓練可使大鼠腦梗死竈週突觸素mRNA和GAP-43 mRNA水平增高,促進運動功能恢複.
목적 연구운동훈련대대서경사조주돌촉소mRNA화생장상관단백(GAP-43)mRNA적표체수평화대서운동공능적영향.방법 장신성고혈압후적대뇌중동맥폐새뇌경사모형대서100지분성훈련조화대조조(n=50),훈련조대서진행운동훈련,대조조대서상규사양.2조대서균우제모성공후제3,7,14천채용횡목행주실험평정각아조대서운동공능,재제모성공후제1,3,7,14,28천시용반정량역전록-다취매련식반응법검측각조대서뇌경사조주변구돌촉소mRNA、GAP-43 mRNA수평.결과 조모후제7,14천,2조대서운동공능득분여본조조모후제3천비교,차이유통계학의의(P<0.01);제모후제7,14천시,훈련조운동공능득분[분별위(3.2±0.3)분화(5.8±0.9)분]현저고우대조조[분별위(1.6±0.4)분화(2.6±0.8)분],차이유통계학의의(P<0.01).2조대서뇌경사조주돌촉소mRNA화GAP-43 mRNA수평균재조모후제1,3,7천시축점증고,차이유통계학의의(P<0.01).훈련조돌촉소mRNA수평재조모후제3,7,14화28천시균고우대조조,차이유통계학의의(P<0.01);훈련조GAP-43 mRNA수평부재조모후제3화제7천시(0.295±0.03、0.512±0.045)고우대조조,차이유통계학의의(P<0.01).결론 운동훈련가사대서뇌경사조주돌촉소mRNA화GAP-43 mRNA수평증고,촉진운동공능회복.
Objective To investigate the effects of mobility training on mobility and the mRNA levels of both synaptophysin and growth associated protein 43 (GAP-43) in the region around an infarction in rats with acute cerebral infarction. Methods Models of cerebral infarction were created in 100 rats through middle cerebral artery occlusion. They were then randomly divided into training and control groups. The motor skill of the rats was examined using a beam walking test. The mRNA levels of both synaptophysin and GAP-43 in the region around the infarction were observed at the 1st, 3rd, 7th, 14th and 28th days after model-creation using a semi-quantitive reverse transcrip-tion polymerase chain reaction. Results The rats' mobility scores increased with training, and significant differ-ences were observed between the average scores of the two groups at the 3rd, 7th and 14th days. The scores were higher in the training group. The mRNA levels of both synaptophysin and GAP-43 in the region around the infarction increased significantly from the 1st to the 3rd and 7th days. Synaptophysin mRNA levels were significantly higher in the trained group at each time point, but the levels of GAP-43 mRNA were significantly higher in the trained group only on the 3rd and 7th days. Conclusions Motor skill and the mRNA levels of synaptophysin and GAP-43 in the region around an infarction can be increased by motor skill training, at least in rats with model acute cerebral infarc-tion.
