CAJ | 학술논문

目的探讨黄芪对快速加减压大鼠急性肾损伤的保护作用及其机制.方法选用健康雄性SD大鼠54只,随机分为3组:(1)对照组(C组)18只,不进行任何处理;(2)快速加减压组(F组)18只,进行快速加减压处理;(3)黄芪预处理组(H组)18只,快速加减压前用10 g/kg黄芪注射液灌胃.分别于快速加减压1、2、12 h后检测血清肌酐(SCr)、肾组织SOD、肾组织丙二醛(MDA),观察快速加减压1 h后.肾组织的病理变化.结果 F组和H组每个时间点SCr水平高于C组(P<0.01),组内2 h SCr水平均高于1、12 h(P<0.01);H组SCr水平在相应时点低于F组(P<0.01);F组和H组每个时间点肾组织MDA水平高于C组(P<0.01),组内2 h水平均高于1、12 h(P<0.01);H组在相应时点低于F组(P<0.01);F组和H组每个时间点肾组织SOD水平低于C组(P<0.01),组内2 h水平均低于1、12h(P<0.01),H组在相应时点高于F组(P<0.01).结论黄芪对快速加减压后肾脏损伤具有保护功能,其机制可能是减少了体内MAD的产生和SOD的消耗.
목적 탐토황기대쾌속가감압대서급성신손상적보호작용급기궤제.방법 선용건강웅성SD대서54지,수궤분위3조:(1)대조조(C조)18지,불진행임하처리;(2)쾌속가감압조(F조)18지,진행쾌속가감압처리;(3)황기예처리조(H조)18지,쾌속가감압전용10 g/kg황기주사액관위.분별우쾌속가감압1、2、12 h후검측혈청기항(SCr)、신조직SOD、신조직병이철(MDA),관찰쾌속가감압1 h후.신조직적병리변화.결과 F조화H조매개시간점SCr수평고우C조(P<0.01),조내2 h SCr수평균고우1、12 h(P<0.01);H조SCr수평재상응시점저우F조(P<0.01);F조화H조매개시간점신조직MDA수평고우C조(P<0.01),조내2 h수평균고우1、12 h(P<0.01);H조재상응시점저우F조(P<0.01);F조화H조매개시간점신조직SOD수평저우C조(P<0.01),조내2 h수평균저우1、12h(P<0.01),H조재상응시점고우F조(P<0.01).결론 황기대쾌속가감압후신장손상구유보호공능,기궤제가능시감소료체내MAD적산생화SOD적소모.
Objective To investigate protective effects and mechanism of astragalus preparation on renal damage induced by fast compression and decompression in rats.Methods 54 SD rats were randomly divided into 3 groups,i.e.the control group consisting of 18 rats without any treatment;the fast compression and decompression group ( fast group) consisting of the same number of rats were exposed to fast compression and decompression;The astragahs experimental group of 18 rats were orally administrated with astragalns preparation (10 g/kg) before and after fast compression and decompression.SCr,SOD and MDA in the renal tissue were measured 1 h,2 h and 12 h following fast compression and decompression.Pathological changes in the renal tissue were observed 1 h after fast compression and decompression.Results SCr and MDA in renal tissue in the fast and experimental groups were higher than that of the control group ( P < 0.01 ).SCr and MDA in the renal tissue in the experimental group were lower than those of the fast group ( P <0.01 ).SCr and MDA in the renal tissue after 2 h were higher after 1 and 12 h within group (P <0.01);SOD in the renal tissue in fast and experimental groups was lower than that of the control group ( P < 0.01 ).SOD in the renal tissue for the experimental group was higher than that of the fast group at all time points(P <0.01 ).SOD in the renal tissue after 2 h was lower than the 1 and 12 h within group (P<0.01).Conclusions Astragalus preparation might be effective against renal damage following fast compression and decompression.Its mechanism might be due to reduced production of MAD and consumption of SOD.