中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2010年
6期
678-681
,共4页
刘合智%白雪薇%王海峰%胡乐乐%周松%杨晓燕%杜国义%杨顺林%史献明%李玉贵
劉閤智%白雪薇%王海峰%鬍樂樂%週鬆%楊曉燕%杜國義%楊順林%史獻明%李玉貴
류합지%백설미%왕해봉%호악악%주송%양효연%두국의%양순림%사헌명%리옥귀
胶体金%色谱法%薄层%耶尔森菌%鼠疫%抗原
膠體金%色譜法%薄層%耶爾森菌%鼠疫%抗原
효체금%색보법%박층%야이삼균%서역%항원
Gold colloid%Chromatography,thin layer%Yersiniapestis%Antigens
目的 研究胶体金免疫层析试纸条检测鼠疫F1抗原的敏感性和特异性.方法通过鼠疫F1抗原单克隆抗体(F1MAb)捕捉F1抗原的胶体金免疫层析(GICA)试纸条,检测308只强毒鼠疫耶尔森菌(鼠疫菌)感染鼠脏器标本和225份对照鼠(达乌尔黄鼠217只,小白鼠5只,豚鼠3只)标本,同时用鼠疫反向间接血球凝集试验(RIHA)微量法和细菌培养做平行检测.结果 225只对照鼠鼠疫细菌学检验及GICA和RIHA的F1抗原检测均为阴性,GICA和RIHA在1×108 cfu/ml水平上未发现与近缘假结核耶尔森菌有交叉反应.GICA、RIHA对鼠疫菌检测灵敏性为2.5×105、2.0×105cfu/ml;检测F1抗原灵敏性为1、10 μg/L.GICA与细菌学检验符合率为97.94%(522/533),Kappa值=0.959,阳性检出率组间比较,差异无统计学意义(x2=0.36,P>0.05);与RIHA符合率为97.94%(522/533),Kappa值=0.959,阳性检出率组间比较差异有统计学意义(x2=9.09,P<0.05).308只实验鼠样本细菌培养阳性284只,284份细菌培养阳性标本中,GICA有280份检测阳性,阳性率为98.59%,高于RIHA[阳性率为96.13%(522/533)],两组比较差异有统计学意义(x2=5.14;P<0.05).GICA的敏感度为98.59%(280/284),特异度为97.19%(242/249),阳性预测值为97.56%(280/287),阴性预测值为98.37%(242/246),Youden指数为0.9578.结论 GICA检测鼠疫F1抗原敏感特异,快速简便,是鼠疫早期快速诊断中有应用价值的检测技术.
目的 研究膠體金免疫層析試紙條檢測鼠疫F1抗原的敏感性和特異性.方法通過鼠疫F1抗原單剋隆抗體(F1MAb)捕捉F1抗原的膠體金免疫層析(GICA)試紙條,檢測308隻彊毒鼠疫耶爾森菌(鼠疫菌)感染鼠髒器標本和225份對照鼠(達烏爾黃鼠217隻,小白鼠5隻,豚鼠3隻)標本,同時用鼠疫反嚮間接血毬凝集試驗(RIHA)微量法和細菌培養做平行檢測.結果 225隻對照鼠鼠疫細菌學檢驗及GICA和RIHA的F1抗原檢測均為陰性,GICA和RIHA在1×108 cfu/ml水平上未髮現與近緣假結覈耶爾森菌有交扠反應.GICA、RIHA對鼠疫菌檢測靈敏性為2.5×105、2.0×105cfu/ml;檢測F1抗原靈敏性為1、10 μg/L.GICA與細菌學檢驗符閤率為97.94%(522/533),Kappa值=0.959,暘性檢齣率組間比較,差異無統計學意義(x2=0.36,P>0.05);與RIHA符閤率為97.94%(522/533),Kappa值=0.959,暘性檢齣率組間比較差異有統計學意義(x2=9.09,P<0.05).308隻實驗鼠樣本細菌培養暘性284隻,284份細菌培養暘性標本中,GICA有280份檢測暘性,暘性率為98.59%,高于RIHA[暘性率為96.13%(522/533)],兩組比較差異有統計學意義(x2=5.14;P<0.05).GICA的敏感度為98.59%(280/284),特異度為97.19%(242/249),暘性預測值為97.56%(280/287),陰性預測值為98.37%(242/246),Youden指數為0.9578.結論 GICA檢測鼠疫F1抗原敏感特異,快速簡便,是鼠疫早期快速診斷中有應用價值的檢測技術.
목적 연구효체금면역층석시지조검측서역F1항원적민감성화특이성.방법통과서역F1항원단극륭항체(F1MAb)포착F1항원적효체금면역층석(GICA)시지조,검측308지강독서역야이삼균(서역균)감염서장기표본화225빈대조서(체오이황서217지,소백서5지,돈서3지)표본,동시용서역반향간접혈구응집시험(RIHA)미량법화세균배양주평행검측.결과 225지대조서서역세균학검험급GICA화RIHA적F1항원검측균위음성,GICA화RIHA재1×108 cfu/ml수평상미발현여근연가결핵야이삼균유교차반응.GICA、RIHA대서역균검측령민성위2.5×105、2.0×105cfu/ml;검측F1항원령민성위1、10 μg/L.GICA여세균학검험부합솔위97.94%(522/533),Kappa치=0.959,양성검출솔조간비교,차이무통계학의의(x2=0.36,P>0.05);여RIHA부합솔위97.94%(522/533),Kappa치=0.959,양성검출솔조간비교차이유통계학의의(x2=9.09,P<0.05).308지실험서양본세균배양양성284지,284빈세균배양양성표본중,GICA유280빈검측양성,양성솔위98.59%,고우RIHA[양성솔위96.13%(522/533)],량조비교차이유통계학의의(x2=5.14;P<0.05).GICA적민감도위98.59%(280/284),특이도위97.19%(242/249),양성예측치위97.56%(280/287),음성예측치위98.37%(242/246),Youden지수위0.9578.결론 GICA검측서역F1항원민감특이,쾌속간편,시서역조기쾌속진단중유응용개치적검측기술.
Objective To study the sensitivity and specificity of gold-immunochromatography assay (GICA) for detection of Yersiniapestis(Y. pestis ) F1 antigen. Methods Viscera organ(liver and spleen) specimens of 308 mice with virulent Y. pestis infection and 225 control specimens of rats(217 Spermophilus dauricus, 5 mice,3 guinea pigs) were detected by GICA dipstick with monoclonal antibody against plague F1 antigen (F1MAb).Meanwhile, micro-method of reverse indirect hemagglutination assay(RIHA) and bacteria culture were carried out for parallel testing. Results Bacteriological examination of 225 control specimens, and F1 antigen detected with GICA and RIHA were all negative. No cross-reaction with related Yersinia pseudotuberculosis at 1 x 108 cfu/ml level was found in GICA and RIHA. Detection sensitivity of Y. pestis by GICA and RIHA were 2.5 × 105 cfu/ml and 2.0 × 105 cfu/ml, respectively, and of F1 antigen were 1μg/L and 10 μg/L, respectively. Coincidence was 97.94% (522/533) between GICA and bacteriological test, Kappa = 0.959, and the difference was statistically insignificant(x2 = 0.36, P > 0.05); and 97.94%(522/533) between GICA and RIHA, Kappa = 0.959, with statistically significant difference in the positive detection rates(x2 = 9.09, P < 0.05). Out of the 308 infected mice, 284 were positive of plague bacterial cultured, In 284 samples with positive bacterial culture, there were 280 of positive detected by GICA for F1 antigen, positive rate of F1 antigen was 98.59%, higher than that by RIHA[the positive rate of 96.13%(522/533)], with statistically significant difference(x2 = 5.14, P < 0.05). Sensitivity of GICA was 98.59% (280/284), specificity was 97.19% (242/249), positive predictive value (PPV) was 97.56% (280/287),negative predictive value ( NPV ) was 98.37% (242/246), and Youden index was 0.9578. Conclusions GICA is sensitive and specific, fast and simple in detection of F1 antigen of the plague. It's a valuable detection technique for early and rapid diagnosis of plague.