白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2012年
5期
269-272
,共4页
郭亚男%王京琼%孟丹%郭俊明%钟国平%俞文英%高志斌
郭亞男%王京瓊%孟丹%郭俊明%鐘國平%俞文英%高誌斌
곽아남%왕경경%맹단%곽준명%종국평%유문영%고지빈
淋巴瘤,大细胞,弥漫性%微RNA21%实时聚合酶链反应
淋巴瘤,大細胞,瀰漫性%微RNA21%實時聚閤酶鏈反應
림파류,대세포,미만성%미RNA21%실시취합매련반응
Lymphoma,large cell,diffuse%Micro RNA 21%Real-time polymerase chain reaction
目的 研究微小RNA-21(miR-21)在弥漫大B细胞淋巴瘤(DLBCL)中的表达,探讨miR-21表达与DLBCL发生、发展的意义及其与临床病理特征和预后的关系.方法 采用实时荧光定量聚合酶链反应(RT-PCR)检测50例DLBCL存档蜡块和12例正常淋巴结中miR-21的表达,并采用免疫组织化学EnVision法检测bcl-2、p53在DLBCL中的表达.结果 miR-21在DLBCL中高表达,且在非生发中心B细胞样(non-GCB)亚型中高于GCB亚型,50例DLBCL中有18例(36.0％)表达bcl-2,21例(42.0％)表达p53.DLBCL中miR-21表达水平与bcl-2呈负相关(P=0.020),与p53表达、DLBCL临床分期呈正相关( P=0.022;P=0.005 ),miR-21高表达的DLBCL患者3年生存率较miR-21低表达者低.结论 miR-21高表达可能是DLBCL恶性度高的标志,是促进DLBCL肿瘤细胞增殖的重要因素,可能对预后有一定的指导意义.bcl-2、p53可能是miR-21在DLBCL中发挥作用的靶标.
目的 研究微小RNA-21(miR-21)在瀰漫大B細胞淋巴瘤(DLBCL)中的錶達,探討miR-21錶達與DLBCL髮生、髮展的意義及其與臨床病理特徵和預後的關繫.方法 採用實時熒光定量聚閤酶鏈反應(RT-PCR)檢測50例DLBCL存檔蠟塊和12例正常淋巴結中miR-21的錶達,併採用免疫組織化學EnVision法檢測bcl-2、p53在DLBCL中的錶達.結果 miR-21在DLBCL中高錶達,且在非生髮中心B細胞樣(non-GCB)亞型中高于GCB亞型,50例DLBCL中有18例(36.0％)錶達bcl-2,21例(42.0％)錶達p53.DLBCL中miR-21錶達水平與bcl-2呈負相關(P=0.020),與p53錶達、DLBCL臨床分期呈正相關( P=0.022;P=0.005 ),miR-21高錶達的DLBCL患者3年生存率較miR-21低錶達者低.結論 miR-21高錶達可能是DLBCL噁性度高的標誌,是促進DLBCL腫瘤細胞增殖的重要因素,可能對預後有一定的指導意義.bcl-2、p53可能是miR-21在DLBCL中髮揮作用的靶標.
목적 연구미소RNA-21(miR-21)재미만대B세포림파류(DLBCL)중적표체,탐토miR-21표체여DLBCL발생、발전적의의급기여림상병리특정화예후적관계.방법 채용실시형광정량취합매련반응(RT-PCR)검측50례DLBCL존당사괴화12례정상림파결중miR-21적표체,병채용면역조직화학EnVision법검측bcl-2、p53재DLBCL중적표체.결과 miR-21재DLBCL중고표체,차재비생발중심B세포양(non-GCB)아형중고우GCB아형,50례DLBCL중유18례(36.0％)표체bcl-2,21례(42.0％)표체p53.DLBCL중miR-21표체수평여bcl-2정부상관(P=0.020),여p53표체、DLBCL림상분기정정상관( P=0.022;P=0.005 ),miR-21고표체적DLBCL환자3년생존솔교miR-21저표체자저.결론 miR-21고표체가능시DLBCL악성도고적표지,시촉진DLBCL종류세포증식적중요인소,가능대예후유일정적지도의의.bcl-2、p53가능시miR-21재DLBCL중발휘작용적파표.
Objective To investigate the expression of miR-21 in diffuse large B cell lymphoma (DLBCL) and normal lymph tissues and its potential relevance with clinicopathological characteristics.Methods The expression levels of miR-21 in 50 primary DLBCL and 12 normal lymph node tissue specimens were examined by TaqMan real-time polymerase chain reaction.The expression of bcl-2 and p53 was detected by immunohistochemistry staining. Results The expression of miR-21 was significantly higher in tumor tissues than that in normal tissues, in GCB subtypes higher than in non-GCB subtypes. And it was negatively correlated with bcl-2 (P=0.020),while positively correlated with p53 (P=0.022). Up-regulated miR-21 expression was low in three years of survival rate. Conclusion MiR-21 may indicate a more aggressive phenotype and serve as a molecular prognostic marker in DLBCL. High-expression of miR-21 is a key feature that is correlated with cell proliferation in DLBCL.miR-21 may have some guiding significance in prognosis.bcl-2,p53 is possibly one of the targets of miR-21 in DLBCL.
