CAJ | 학술논문

用空肠弯曲菌免疫的BALB/C小鼠脾细胞与NS-1骨髓瘤细胞融合,获得10株能稳定分泌抗空肠弯曲菌单克隆抗体的杂交瘤细胞株.这些单克隆抗体与空肠弯曲菌活菌做玻片凝集试验,呈强阳性反应;与福尔马林灭活的空肠弯曲菌做试管凝集试验,均为阴性结果,而与煮沸后的菌体做试验亦呈阳性反应.这些单克隆抗体在体外对空肠弯曲菌无杀菌作用.
용공장만곡균면역적BALB/C소서비세포여NS-1골수류세포융합,획득10주능은정분비항공장만곡균단극륭항체적잡교류세포주.저사단극륭항체여공장만곡균활균주파편응집시험,정강양성반응;여복이마림멸활적공장만곡균주시관응집시험,균위음성결과,이여자비후적균체주시험역정양성반응.저사단극륭항체재체외대공장만곡균무살균작용.
Spleen cells of BALB/c mice immunized with formalinized Campylobacter jejuni were fused with NS-I myeloma cells,and antibodies in the cell culture supernatants and ascites were detected by ELISA.Ten hybridoma cell lines secreting monoclonal antibodies against Campylobacter jejuni were obtained.These antibodies reaoted strongly in slide agglutination tests with live Campylobaoter and none of them reaoted in tube agglutination tests with formalinized whole bacteria,but agglutinated with boiled bacteria.None of the monoclonal antibodies reacted with unrelated enterobacteria which have been tested.These monoclonal antibodies displayed insignificant bactericidal effect on Campylobacter jejuni in vitro.