中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
8期
461-464
,共4页
祝成亮%李艳%闫晓红%童永清%汪明%夏尊恩%刘芳
祝成亮%李豔%閆曉紅%童永清%汪明%夏尊恩%劉芳
축성량%리염%염효홍%동영청%왕명%하존은%류방
SARS病毒%核壳蛋白质类%环氧化酶2%基因表达
SARS病毒%覈殼蛋白質類%環氧化酶2%基因錶達
SARS병독%핵각단백질류%배양화매2%기인표체
SARS virus%Nucleocapsid protein%Cyclooxygenase 2%Gene expression
目的 探讨严重急性呼吸综合征冠状病毒(SARS-CoV)核衣壳蛋白对环氧合酶-2(COX-2)表达的影响.方法 将带有COX-2基因启动子的报告质粒分别与表达SARS-CoV基因组的单个基因的质粒共转染293T细胞,并测定荧光素酶的活性,采用RT-PCR和Western印迹法检测COX-2 mRNA和蛋白表达的变化.结果 SARS-CoV核衣壳蛋白能显著激活COX-2基因启动子的活性,并在mRNA和蛋白水平上调COX-2的表达,且这种激活作用随着核衣壳蛋白浓度的增加而增强.结论 SARS-CoV核衣壳蛋白能特异性地激活COX-2的表达.
目的 探討嚴重急性呼吸綜閤徵冠狀病毒(SARS-CoV)覈衣殼蛋白對環氧閤酶-2(COX-2)錶達的影響.方法 將帶有COX-2基因啟動子的報告質粒分彆與錶達SARS-CoV基因組的單箇基因的質粒共轉染293T細胞,併測定熒光素酶的活性,採用RT-PCR和Western印跡法檢測COX-2 mRNA和蛋白錶達的變化.結果 SARS-CoV覈衣殼蛋白能顯著激活COX-2基因啟動子的活性,併在mRNA和蛋白水平上調COX-2的錶達,且這種激活作用隨著覈衣殼蛋白濃度的增加而增彊.結論 SARS-CoV覈衣殼蛋白能特異性地激活COX-2的錶達.
목적 탐토엄중급성호흡종합정관상병독(SARS-CoV)핵의각단백대배양합매-2(COX-2)표체적영향.방법 장대유COX-2기인계동자적보고질립분별여표체SARS-CoV기인조적단개기인적질립공전염293T세포,병측정형광소매적활성,채용RT-PCR화Western인적법검측COX-2 mRNA화단백표체적변화.결과 SARS-CoV핵의각단백능현저격활COX-2기인계동자적활성,병재mRNA화단백수평상조COX-2적표체,차저충격활작용수착핵의각단백농도적증가이증강.결론 SARS-CoV핵의각단백능특이성지격활COX-2적표체.
Objective To investigate the effect of nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) on the expression of cyclooxygenase-2 (COX-2). Methods 293T cells were co-transfected with reporter plasmid pCOX-2-Luc containing the luciferase gene under the control of COX-2 promoter and plasmids carrying individual genes of SARS-CoV, and luciferase activity was measured. Expression of COX-2 mRNA and protein was measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results N protein of SARS-CoV enhanced COX-2 gene promoter activity, and upregulated COX-2 mRNA expression.COX-2 protein production in 293T cells was N protein concentration-dependent. Conclusion N protein of SARS-CoV could specifically activate COX-2 expression.
