CAJ | 학술논문

目的探讨低温、地塞米松因素对大鼠睾丸扭转复位后的保护作用以及对生殖细胞凋亡的影响.方法本实验采用2×2析因设计.50只健康青春期SD雄性大鼠随机分成五组:A组为扭转组;B组为扭转加低温组;C组扭转加激素组;D组扭转加激素加低温组;E组对照组.每组10只.建立单侧睾丸扭转模型.每组根据设定处理方式的不同分别进行处理.复位后36 h,采集左侧睾丸并行生化指标(SOD,CAT活性及MDA含量)检测;免疫组织化学法检测生精细胞凋亡指数(AI),光镜下观察睾丸组织学变化.结果低温因素与地塞米松因素之间有交互效应.低温、地塞米松因素单独作用时对扭转睾丸有保护作用.结论低温和地塞米松共同作用能更好的减少生精细胞的凋亡,提高抗氧化酶系统的抗氧化能力,改善扭转睾丸的生存能力.
목적 탐토저온、지새미송인소대대서고환뉴전복위후적보호작용이급대생식세포조망적영향.방법 본실험채용2×2석인설계.50지건강청춘기SD웅성대서수궤분성오조:A조위뉴전조;B조위뉴전가저온조;C조뉴전가격소조;D조뉴전가격소가저온조;E조대조조.매조10지.건립단측고환뉴전모형.매조근거설정처리방식적불동분별진행처리.복위후36 h,채집좌측고환병행생화지표(SOD,CAT활성급MDA함량)검측;면역조직화학법검측생정세포조망지수(AI),광경하관찰고환조직학변화.결과 저온인소여지새미송인소지간유교호효응.저온、지새미송인소단독작용시대뉴전고환유보호작용.결론 저온화지새미송공동작용능경호적감소생정세포적조망,제고항양화매계통적항양화능력,개선뉴전고환적생존능력.
Objective To investigate the protective effects of hypothermia, dexamethasone on torsion/detorsion testis and the influence of apoptosis germ cells in rats by different treatment on left torsion testis of rat models. Methods 50 healthy male Sprague-Dawley rats were randomly divided into five equal groups: A ( torsion), B ( torsion + hypothermia), C ( torsion + ), D ( torsion + hypothermia + dexamethasone ) and E (control).Group A, B, C and D were submitted to unilateral 720° testicular torsion. Every group was differently treated according to different set methods and detorsion were done after 2 hours. 36 hours later, they were killed and the left testis was took out and then its biochemical indicator was examined with refer to the superoxide dismutase (SOD); the activity of catalase(CAT) and the level of malonic diethylaldehyde(MDA). Germ cell apoptosis index was detected by immunohistochemistry. The histological changes were observed by light microscope. Results Compared with single fact, its protective effects all have statistical significance, among which there is interaction between hypothermia and dexamethasone facts. When one of them act on torsion testis singly, hypothermia and dexamethasone all have protective effect on it. Conclusion Can increase survivability of the torsional testicle during ischemia time and reduce the germ cell apoptosis and repress the generation of oxygen-derived free radicals and peroxidatic reaction of lipid. can attenuate reperfusion damage and infiltration/release effects of inflammatory cells of the torsion/detorsion testis. The results are better when combined together. Hypothermia Dexamethasone