中华（上海）骨髓库的HLA分布报告
중화（상해）골수고적HLA분포보고
Report of HLA distribution of Chinese Bone Marrow Bank(Shanghai)
  • 万方数据
    中华器官移植杂志 중화기관이식잡지
    CHINESE JOURNAL OF ORGAN TRANSPLANTATION
    2001年 3期 179-185 ,共7页

    张工梁%杨颖%冯喆%张雁征%冯明亮%嵇月华%陆琼%马俊%季芸%毛昕%张钦辉%杨钧仪%张瑾%袁惠章

    장공량%양영%풍철%장안정%풍명량%혜월화%륙경%마준%계예%모흔%장흠휘%양균의%장근%원혜장

    骨髓%生物标本库%HLA抗原%基因频率%数据收集 骨髓%生物標本庫%HLA抗原%基因頻率%數據收集
    골수%생물표본고%HLA항원%기인빈솔%수거수집
    目的报告中华（上海）骨髓库中HLA血型资料，了解中国人群HLA分布特点。方法用微量淋巴细胞毒试验检测11 995名志愿者的HLA-A、B抗原，并用序列特异引物聚合酶链反应（PCR-SSP）进行DNA水平的质控和对有疑问结果作复查。结果共检出A座位特异性25种，B座位抗原特异性52种，有显著意义的连锁不平衡单型有62种，其中以A2-B46、A30-B13、A33-B58、A11.1-B60、A24-B62、A24-B60等为最常见；检索HLA-A、B、DR抗原（基因）匹配志愿者的“命中率”约为1‰。结论中国人群HLA分布有自身特点，而现有骨髓库HLA-A、B基因的遗传特征介于南、北方汉族之间，但偏向于北方人群。
    목적보고중화（상해）골수고중HLA혈형자료，료해중국인군HLA분포특점。방법용미량림파세포독시험검측11 995명지원자적HLA-A、B항원，병용서렬특이인물취합매련반응（PCR-SSP）진행DNA수평적질공화대유의문결과작복사。결과공검출A좌위특이성25충，B좌위항원특이성52충，유현저의의적련쇄불평형단형유62충，기중이A2-B46、A30-B13、A33-B58、A11.1-B60、A24-B62、A24-B60등위최상견；검색HLA-A、B、DR항원（기인）필배지원자적“명중솔”약위1‰。결론중국인군HLA분포유자신특점，이현유골수고HLA-A、B기인적유전특정개우남、북방한족지간，단편향우북방인군。
    Objective　To study the characteristic of HLA distribution of Chinese Bone Marrow Bank(Shanghai),this paper analyses the HLA distribution of a population of 11 995 persons which were major part of this Bank. Methods　HLA-A、B antigen of volunteers were detected by MLT(microlymphocytetotoxicity test) method, and at the same time, some samples were selected as control and their AB locus were typed at the DNA level by PCR-SSP method, ambiguous samples were checked by PCR-SSO also.Results　HLA-A、B typing results of 11 995 volunteers were gotten, and their antigen frequency, gene frequency, haplotype frequency and linkage disequilibrium parameter were gotten also.Conclusions　the HLA-A、B distribution of this population its own characters, according to these characters, we can induce Chines Bone Marrow Bank(Shanghai) were mainly between the south and the north han-population,a little closer to the northern. There are gotten more than 60 binds haplotypes which had significant linkage disequili-brium sense,among them,the most common were A2-B46,A30-B13,A33-B58,A11.1-B60,A24-B62,A24-B60.And the chance of finding out a matched donor from this Bank is roughly one-thousandth.
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