CAJ | 학술논문

目的应用病例对照分析法对骨骼肌糖原相关蛋白磷酸酶1的糖原靶调节亚单位基因多态性与2型糖尿病相关性进行研究。方法应用寡核苷酸连接测定技术和直接电泳的方法，对该基因的Asp905Tyr和3'非翻译区ARE多态性位点基因型进行了测定。结果 BMI25携带Asp905等位基因组能增加2型糖尿病的危险（OR=3.66; 95% CI: 1.48-9.06）；ARE多态性与2型糖尿病未见相关(OR=1.15; 95% CI: 0.62-2.14)。结论 Asp905协同肥胖增加2型糖尿病的危险。在中国人群中，该基因的Asp905Tyr和ARE多态性不是致糖尿病基因主要的变异。
목적응용병례대조분석법대골격기당원상관단백린산매1적당원파조절아단위기인다태성여2형당뇨병상관성진행연구。방법응용과핵감산련접측정기술화직접전영적방법，대해기인적Asp905Tyr화3'비번역구ARE다태성위점기인형진행료측정。결과 BMI25휴대Asp905등위기인조능증가2형당뇨병적위험（OR=3.66; 95% CI: 1.48-9.06）；ARE다태성여2형당뇨병미견상관(OR=1.15; 95% CI: 0.62-2.14)。결론 Asp905협동비반증가2형당뇨병적위험。재중국인군중，해기인적Asp905Tyr화ARE다태성불시치당뇨병기인주요적변이。
Objective To detect the relationship between the polymorphism of the glycogen-targeting regulatory subunit of the skeletal muscle glycogen-associated protein phosphatase 1 (PPP1R3) gene and type 2 diabetes by case-control study. Methods We genotyped the PPP1R3 gene Asp905Tyr polymorphism and a common 3'-untranslated region AT (AU)-rich element (ARE) polymorphism in 101 type 2 diabetic patients and 101controls by oligonucleotide ligation assay (OLA) and polyacrylamide gel elecrophoresis, respectively. Results Subjects with Tyr/Tyr genotypes whose body mass index (BMI)<25 were used as the reference group. Those whose BMI25 with Asp905 had a 3.66-fold increase (95% CI: 1.48-9.06, P=0.005) in type 2 diabetes risk. No association was found between 3'UTR ARE polymorphism and type 2 diabetes mellitus (OR=1.15; 95% CI: 0.62-2.14, P=0.65). Conclusion A joint effect between the Asp905 and BMI increases the risk of type 2 diabetes, and Asp905Tyr and ARE polymorphism of PPP1R3 gene are not the major diabetogenic gene variants in Chinese population.