CAJ | 학술논문

目的克隆肾癌相关新基因GYLZ-RCC18的全长,并探讨其功能.方法应用SMART RACE技术克隆GYLZ-RCC18的全长,应用逆转录PCR的方法检测其在肾癌组织和细胞系中的表达特点,以及与肾癌组织分级、分期的关系,通过转染GYLZ-RCC18的反义寡核苷酸阻断其表达,观察其对肾癌细胞系生命特征的变化.结果 GYLZ-RCC18全长约3.5 kb(基因号:BE825133).GYLZ-RCC18在肾癌特异高表达,正常肾表达量极低甚至不表达,两者表达量差别1-9倍之间;GYLZ-RCC18在高分级、高分期肾癌中表达明显高于低分级、低分期肾癌的表达.转染反义基因可明显抑制GRC-1的生长、降低其增殖活性、减少核分裂、并诱导其持续性凋亡.结论本研究表明GYLZ-RCC18是肾癌发生发展密切相关的重要新的癌基因,它的表达与肾癌细胞的生存、增殖、抗凋亡发展密切相关,它的成功克隆为今后肾癌分子机制研究提供了新的理论依据.
목적극륭신암상관신기인GYLZ-RCC18적전장,병탐토기공능.방법응용SMART RACE기술극륭GYLZ-RCC18적전장,응용역전록PCR적방법검측기재신암조직화세포계중적표체특점,이급여신암조직분급、분기적관계,통과전염GYLZ-RCC18적반의과핵감산조단기표체,관찰기대신암세포계생명특정적변화.결과 GYLZ-RCC18전장약3.5 kb(기인호:BE825133).GYLZ-RCC18재신암특이고표체,정상신표체량겁저심지불표체,량자표체량차별1-9배지간;GYLZ-RCC18재고분급、고분기신암중표체명현고우저분급、저분기신암적표체.전염반의기인가명현억제GRC-1적생장、강저기증식활성、감소핵분렬、병유도기지속성조망.결론본연구표명GYLZ-RCC18시신암발생발전밀절상관적중요신적암기인,타적표체여신암세포적생존、증식、항조망발전밀절상관,타적성공극륭위금후신암분자궤제연구제공료신적이론의거.
Objective To clone the full length of renal cell carcinoma (RCC) related novel gene GYLZ -RCC18 and study its function.Methods SMART RACE technology was used to clone the full length of GYLZ-RCC18. RT-PCR was used to detect its expression in renal cell carcinoma tissue at different s tages and grades. We transfected the antisense oligonucleotide of GYLZ-RCC18 t o renal cell carcinoma cell line, GRC-1, and analyzed proliferation activity, g rowth rate, apoptosis, and mortality changes.Results The full length of GYLZ-RCC18 (GenBank accession number: BE825133) cDNA was abo ut 3.5kb. GYLZ-RCC18 had a higher expression in higher grades and stage s of renal cell carcinoma than in lower ones. The expression of GYLZ-RCC18 in rena l cell carcinoma was much higher than in normal kidney. After the transfection of GYLZ-RCC18 antisense oligonucleotide, the mortality of GRC-1 increased sig nificantly, while proliferative activity and growth rate were substantially inhi bited at the same time. The antisense oligonucleotide induced apoptosis of GRC -1 through the entire observation time.Conclusion GYLZ-RCC18 is an important novel gene related to renal cell carcinoma. Overexp ression of this gene results in higher growth and proliferative activity and has an antiapoptosis effect on renal cell carcinoma cells. Transfection of the ant isense oligonucleotide may inhibit the generation and development of renal cell carcinoma.