CAJ | 학술논문

目的评价细粒棘球蚴纯化囊液粗抗原、天然抗原B(nAgB)及其3个重组亚基检测囊型包虫病的效能.方法从新疆源细粒棘球蚴原头节中提取总RNA,通过RT PCR获得编码AgB8/1、AgB8/2和AgB8/3基因片段并克隆入pGEX3X表达载体,IPTG诱导表达分别得到重组蛋白rAgB8/1、rAgB8/2、rAgB8/3;羊细粒棘球蚴囊液经透析沉淀获得纯化囊液粗抗原(HCF)、此纯化囊液粗抗原经煮沸离心弃沉淀得到天然抗原B.用制备的5种抗原分别作为包被抗原,以ELISA法检测囊型包虫病人及其它寄生虫病人和健康人血样中抗体.结果 HCF、nAgB、rAgB8/1、rAgB8/2和rAgB8/3应用于ELISA法检测囊型包虫病人血清的敏感度依次为90.8％(79/87),87.4％(76/87),67.8％(59/87),78.2％(68/87)和59.8％(52/87)；特异度依次为96.0％,96.0％,96.0％,98.0％和97.0％.纯化HCF、nAgB、rAgB8/1、rAgB8/2和rAgB8/3的诊断效能分别为93.5％,91.9％,82.8％,88.7％和79.6％.纯化HCF和nAgB检测的敏感度高于rAgB8/1、rAgB8/2和rAgB8/3(P＜0.05)；rAgB8/2检测的敏感度高于rAgB8/1和rAgB8/3(P＜0.05);5种抗原检测的特异度之间均无统计学差异(P＞0.05).结论天然抗原应用ELISA法检测囊型包虫病的效能总体优于重组抗原.
목적 평개세립극구유순화낭액조항원、천연항원B(nAgB)급기3개중조아기검측낭형포충병적효능.방법종신강원세립극구유원두절중제취총RNA,통과RT PCR획득편마AgB8/1、AgB8/2화AgB8/3기인편단병극륭입pGEX3X표체재체,IPTG유도표체분별득도중조단백rAgB8/1、rAgB8/2、rAgB8/3;양세립극구유낭액경투석침정획득순화낭액조항원(HCF)、차순화낭액조항원경자비리심기침정득도천연항원B.용제비적5충항원분별작위포피항원,이ELISA법검측낭형포충병인급기타기생충병인화건강인혈양중항체.결과 HCF、nAgB、rAgB8/1、rAgB8/2화rAgB8/3응용우ELISA법검측낭형포충병인혈청적민감도의차위90.8％(79/87),87.4％(76/87),67.8％(59/87),78.2％(68/87)화59.8％(52/87)；특이도의차위96.0％,96.0％,96.0％,98.0％화97.0％.순화HCF、nAgB、rAgB8/1、rAgB8/2화rAgB8/3적진단효능분별위93.5％,91.9％,82.8％,88.7％화79.6％.순화HCF화nAgB검측적민감도고우rAgB8/1、rAgB8/2화rAgB8/3(P＜0.05)；rAgB8/2검측적민감도고우rAgB8/1화rAgB8/3(P＜0.05);5충항원검측적특이도지간균무통계학차이(P＞0.05).결론 천연항원응용ELISA법검측낭형포충병적효능총체우우중조항원.