CAJ | 학술논문

通过检测猪肉中呋喃唑酮代谢物的残留以评价试剂盒的性能，采用酶联免疫法对猪肉中的呋喃唑酮代谢物残留量进行测定。结果表明：该方法的线性检测范围为0．025-2．025μg／L，最低检测限为0．1μg／kg，样本添加回收率为78．2％-100.3％，变异系数为4．7％-10-3％，与呋喃唑酮的交叉反应率为16.13％，与其他药物的交叉反应率均小于1％，且对实际样本的检测结果与液相色谱一串联质谱法基本一致。该法灵敏准确、重复性好、特异性高，适用于猪肉中呋喃唑酮代谢物残留的检测。
통과검측저육중부남서동대사물적잔류이평개시제합적성능，채용매련면역법대저육중적부남서동대사물잔류량진행측정。결과표명：해방법적선성검측범위위0．025-2．025μg／L，최저검측한위0．1μg／kg，양본첨가회수솔위78．2％-100.3％，변이계수위4．7％-10-3％，여부남서동적교차반응솔위16.13％，여기타약물적교차반응솔균소우1％，차대실제양본적검측결과여액상색보일천련질보법기본일치。해법령민준학、중복성호、특이성고，괄용우저육중부남서동대사물잔류적검측。
An enzyme-linked immunosorbent assay （ELISA） was proposed to determine residual furazolidone metabolite in pork using a commercially available ELISA kit. The results showed that the sensitivity and detection limit of the assay were 0.025-- 2.025 μg/L and 0.1 μg/kg, respectively. The recoveries of standard additions of furazolidone metabolite averaged 78.2% -- 100.3% across three levels （n = 3）, and the coefficients of variation were 4.7%--10.3%. The cross-reactivity towards furazolidone was 16.3%; as for other drags investigated, the cross-reactivity was below 1%. Analytical results obtained using the assay were in substantial accordance with those from liquid chromatographic tandem mass spectrometry （LC-MS/MS） determination. The assay was sensitive, accurate, repeatable, specific and suitable for the determination of furazolidone metabolite residue in pork.