CAJ | 학술논문

通过大肠杆菌和金黄色葡萄球菌BBF的模型，考察肉桂酸、连翘苷、大黄素、亚硫酸穿心莲内酯对BBF形成的影响。在37℃下将细菌培养24h使其形成BBF后加入样品继续培养24h，用结晶紫染色。通过测量吸光度来考察单体对BBF的影响。结果表明，大黄素、连翘苷、肉桂酸、亚硫酸穿心莲内酯（浓度为1．000mg／mL）对金黄色葡萄球菌BBF的抑制率分别为67．26％、10．91％、18．26％、17．05％；对大肠杆菌BBF的抑制率分别为32。99％、36．13％、49．79％、17．05％。由此可见，大黄素对金黄色葡萄球菌BBF的抑制作用较强，肉桂酸对大肠杆菌BBF的抑制作用较强。
통과대장간균화금황색포도구균BBF적모형，고찰육계산、련교감、대황소、아류산천심련내지대BBF형성적영향。재37℃하장세균배양24h사기형성BBF후가입양품계속배양24h，용결정자염색。통과측량흡광도래고찰단체대BBF적영향。결과표명，대황소、련교감、육계산、아류산천심련내지（농도위1．000mg／mL）대금황색포도구균BBF적억제솔분별위67．26％、10．91％、18．26％、17．05％；대대장간균BBF적억제솔분별위32。99％、36．13％、49．79％、17．05％。유차가견，대황소대금황색포도구균BBF적억제작용교강，육계산대대장간균BBF적억제작용교강。
The effects of einnamic acid, phillyrin, emodin and sulfite andrographolide on bacterial biofilm （BBF） formation were studied by BBF model of Escherichia coli and Staphylococcus aureus. When bacteria formed into BBF at 37 ℃ after 24 hours, samples were added and then cultured for 24 hours. The absorbanee was measured to examine the impact of monomers on BBF after crystal violet staining. Results showed that the inhibition rates of emodin, phillyrin, cinnamic acid and sulfite andrographolide against BBF of Staphylococcus aureus under the concentration of 1.000 mg/mL were 67.26%, 10.91%, 18.26% and 17.05%, respectively, and the inhibition rates against BBF of Escherichia coli were 32.99%, 36.13%, 49.79% and 17.05%, respectively. Emodin had stronger inhibition to BBF of Staphylococcus aureus, while cinnamic acid had stronger inhibition to BBF of Escherichia coll.