CAJ | 학술논문

通过采用蛋白质组学方法分析酵母家族蛋白激酶的研究中发现：半胱氨酸残基上存在多种蛋白修饰形式，其中3种可能为蛋白质翻译后修饰，9种可能来自于体外反应，主要由体外的化学衍生反应生成。通过二级质谱数据解析和生物信息学方法，研究了激酶家族蛋白质半胱氨酸残基上修饰的种类和化学结构，以及可能的反应过程，并对这些修饰出现的频率进行了归类分析。
통과채용단백질조학방법분석효모가족단백격매적연구중발현：반광안산잔기상존재다충단백수식형식，기중3충가능위단백질번역후수식，9충가능래자우체외반응，주요유체외적화학연생반응생성。통과이급질보수거해석화생물신식학방법，연구료격매가족단백질반광안산잔기상수식적충류화화학결구，이급가능적반응과정，병대저사수식출현적빈솔진행료귀류분석。
Cysteine has the reductive thiol functional group, The cysteine residues in protein are prone to oxidation and participate in some nuclephilic reactions. With the analytical methods ofproteomics, various modifications are discovered on cysteine, three of which might be the post-translational modifications and nine might be chemical reactions in vitro, especially some chemical derivations. Moreover, we investigated the types and the chemical structures of the modifications, possible reaction processes through MS/MS interpretations combined with bioinformatic analysis, and made statistical classifications on their frequencies.