CAJ | 학술논문

以鸡胚蛋白为底物，通过单因素及正交试验，确定菠萝蛋白酶和胰蛋白酶对鸡胚蛋白水解的最佳工艺。结果表明：菠萝蛋白酶酶解鸡胚蛋白最佳工艺条件为底物质量分数6％、酶解温度55℃、pH5、酶解时间6h、酶与底物质量比（[E／S]）为1．5％，水解度可达45．53％；胰蛋白酶酶解鸡胚蛋白最佳工艺条件为底物质量分数6％、酶解温度55℃、pH5、酶解时间6h、酶与底物质量比（[E／S]）为1．5％，水解度可达38．17％。菠萝蛋白酶和胰蛋白酶在最佳水解条件下水解度分别为45．53％、38．17％，可溶性蛋白含量分别为20．15、22．69mg／mL，多肽含量分别为62．55、50．26mg／mL。菠萝蛋白酶比胰蛋白酶更适宜酶解鸡胚蛋白。
이계배단백위저물，통과단인소급정교시험，학정파라단백매화이단백매대계배단백수해적최가공예。결과표명：파라단백매매해계배단백최가공예조건위저물질량분수6％、매해온도55℃、pH5、매해시간6h、매여저물질량비（[E／S]）위1．5％，수해도가체45．53％；이단백매매해계배단백최가공예조건위저물질량분수6％、매해온도55℃、pH5、매해시간6h、매여저물질량비（[E／S]）위1．5％，수해도가체38．17％。파라단백매화이단백매재최가수해조건하수해도분별위45．53％、38．17％，가용성단백함량분별위20．15、22．69mg／mL，다태함량분별위62．55、50．26mg／mL。파라단백매비이단백매경괄의매해계배단백。
The optimal process conditions for hydrolyzing chicken embryo protein by trypsin or bromelain were determined using one-factor-at-a-time combined with orthogonal array design method. Under the optimal process conditions, the degree of hydrolysis of chicken embryo protein by trypsin and bromelain were 45.53% and 38.17%, and the resulting hydrolyates contained 20.15 mg/mL and 22.69 mg/mL soluble protein and 62.55 mg/mL and 50.26 mg/mL polypeptides, respectively. These results demonstrate that bromelain is more suitable to hydrolyze chicken embryo protein than trypsin.