CAJ | 학술논문

以三氯氧磷（POCl3）作为辅助试剂，进行了酪氨酸（Tyr）自组装成肽反应的研究，并利用液相色谱-质谱联用和电喷雾多级质谱技术对反应体系进行了组分分析和结构鉴定。在体系中不仅鉴定出了Tyr寡肽系列，还检测到了系列磷酰化酪氨酸及磷酰化小肽。这些化合物的检出丰富了肽库，有助于揭示氨基酸成肽反应的机理，并探讨了芳香族肽化合物的质谱裂解规律。
이삼록양린（POCl3）작위보조시제，진행료락안산（Tyr）자조장성태반응적연구，병이용액상색보-질보련용화전분무다급질보기술대반응체계진행료조분분석화결구감정。재체계중불부감정출료Tyr과태계렬，환검측도료계렬린선화락안산급린선화소태。저사화합물적검출봉부료태고，유조우게시안기산성태반응적궤리，병탐토료방향족태화합물적질보렬해규률。
The reaction of tyrosine assembling into peptide directed by POCl3 as auxiliary reagent was studied in detail.Using liquid chromatography-mass spectrometry and electrospray ionization tandem multi-stage mass spectrometry techniques,the structures of products were analyzed and confirmed.In the reaction system of Phe with POCl3,not only Tyr oligopeptide but phosphoryl tyrosine and small phosphoryl peptide were identified.The identification of these chemical compounds enriches peptide library,and contributes to uncover the mechanism which amino acids turn into peptide.And fragmentation pathways of aromatic peptide by MS was discussed as well.