CAJ | 학술논문

对四株不同属的真菌进行单独培养及两两混合培养,观察其长势,分别测定其粗酶液的木聚糖酶活性、纤维素酶活性、燕麦秸秆降解能力,比较不同混合菌组合发酵后,菌之间、酶之间存在的协同作用和拮抗作用.试验结果表明：单菌发酵长势优于混菌发酵;单菌FQ7-31、P647生长最佳,组合FQ7-31与P647生长中拮抗作用最强.单菌发酵产木聚糖酶活性明显高于混菌发酵,T537单菌发酵后木聚糖酶活性最高达49.3U/g,组合P647与T537拮抗作用最强,木聚糖酶活性与长势之间呈负相关.单菌发酵后纤维素酶活性明显低于混菌,因此混菌发酵后纤维素酶活性存在着协同作用,协同作用最强的组合是P647与T537.粗酶液对秸秆的降解作用中,FQ7-31与其他三属真菌之间存在着明显的协同作用.发酵120小时后,A3.3567培养基中还原糖产量最高.
대사주불동속적진균진행단독배양급량량혼합배양,관찰기장세,분별측정기조매액적목취당매활성、섬유소매활성、연맥갈간강해능력,비교불동혼합균조합발효후,균지간、매지간존재적협동작용화길항작용.시험결과표명：단균발효장세우우혼균발효;단균FQ7-31、P647생장최가,조합FQ7-31여P647생장중길항작용최강.단균발효산목취당매활성명현고우혼균발효,T537단균발효후목취당매활성최고체49.3U/g,조합P647여T537길항작용최강,목취당매활성여장세지간정부상관.단균발효후섬유소매활성명현저우혼균,인차혼균발효후섬유소매활성존재착협동작용,협동작용최강적조합시P647여T537.조매액대갈간적강해작용중,FQ7-31여기타삼속진균지간존재착명현적협동작용.발효120소시후,A3.3567배양기중환원당산량최고.
Different genus fungus strains（Aspergillus sp.3.3567,Fusrium sp.Q7-31,Pestalotiopsis sp.M50647 Trichoderma sp.M50537） were cultured singled and mixed two of them together.The growths of the cultivation were observed,crude xylanase、 cellulase、 oat straw degradation activity of cultivations were determined,synergistic and antagonistic effects were studied.The result showed that the singled cultivations were better that mixed one on the growth condition.Those singled culture of strain FQ7-31 and P647 had the best growth,while they cultured together the antagonistic effects are strongest.For the xylanase activity,antagonistic effects are also existed in mixed cultivation compared with singled cultivations.Among all cultivations,the xyalanase activity of strain T537 was highest and reached 49.3U/g after cultivation single.The group which mixed cultivation with P647 and T537 had the strongest antagonistic effect.Xylanase had the minus correlation with growth of fungi.The groups of singled cultivations,which cellulase activities are lowed than the mixed groups;therefore,synergistic effects are existed in mixed cultivation compared with singled cultivations on cellulase.Among all cultivations,the group of strongest synergistic effect for cellulase was the group of T537 and P647.After mixed cultivation,the groups mixed with FQ7-31 had the obvious synergistic effects.After 120 hours cultivation,the reducing sugar of the singled cultivitation of A3.3567 reached highest.