陕西师范大学学报（自然科学版）
陝西師範大學學報（自然科學版）
협서사범대학학보（자연과학판）
JOURNAL OF SHAANXI NORMAL UNIVERSITY(NATURAL SCIENCE EDITION)
2010年
5期
77-81
,共5页
丹参%COR413基因%实时定量PCR
丹參%COR413基因%實時定量PCR
단삼%COR413기인%실시정량PCR
Salvia miltiorrhiza Bunge%Cold-regulated gene%Real-time PCR
从丹参EST数据库筛选出一条与冷调节基因（cold-regulated gene,COR）家族同源性较高的基因序列,并利用PCR方法得到该基因的DNA序列,命名为SmCOR413.该基因DNA序列长1708 bp,包含4个外显子和3个内含子,编码171个氨基酸,与其他9种植物中的COR413高度相似,相似性介于66%~78%之间,推测为COR413基因家族的一个新基因.生物信息学分析表明,SmCOR413所编码蛋白的分子量为19.77 kD,理论等电点为8.95,不具备信号肽.实时定量PCR检测的结果显示,SmCOR413在丹参根、茎和叶中都有表达,在叶、茎中表达量较高,根部的表达量最低.
從丹參EST數據庫篩選齣一條與冷調節基因（cold-regulated gene,COR）傢族同源性較高的基因序列,併利用PCR方法得到該基因的DNA序列,命名為SmCOR413.該基因DNA序列長1708 bp,包含4箇外顯子和3箇內含子,編碼171箇氨基痠,與其他9種植物中的COR413高度相似,相似性介于66%~78%之間,推測為COR413基因傢族的一箇新基因.生物信息學分析錶明,SmCOR413所編碼蛋白的分子量為19.77 kD,理論等電點為8.95,不具備信號肽.實時定量PCR檢測的結果顯示,SmCOR413在丹參根、莖和葉中都有錶達,在葉、莖中錶達量較高,根部的錶達量最低.
종단삼EST수거고사선출일조여랭조절기인（cold-regulated gene,COR）가족동원성교고적기인서렬,병이용PCR방법득도해기인적DNA서렬,명명위SmCOR413.해기인DNA서렬장1708 bp,포함4개외현자화3개내함자,편마171개안기산,여기타9충식물중적COR413고도상사,상사성개우66%~78%지간,추측위COR413기인가족적일개신기인.생물신식학분석표명,SmCOR413소편마단백적분자량위19.77 kD,이론등전점위8.95,불구비신호태.실시정량PCR검측적결과현시,SmCOR413재단삼근、경화협중도유표체,재협、경중표체량교고,근부적표체량최저.
One sequence which showed homology with COR413（cold-regulated gene）gene family was found from the expressed sequence tags（EST） of Salvia miltiorrhiza using the BLAST approach.The full-length cDNA and DNA sequence of the gene was cloned by PCR and named as SmCOR413.The DNA sequence was 1708bp, and contained four extrons and three introns.It encoded a predicated polypeptide of 171 amino acids with a molecular weight of 19.77 kDa and a predicated pI of 8.95.Amino acids similarity of SmCOR413 compared with COR413 in other nine plants reached 66%—78%.This gene maybe belonged to COR413 gene family without signal peptide.Real-time PCR result showed that the SmCOR413 expressed in root,stem and leaf.The mRNA of SmCOR413 was the most abundant in leaf and stem,the least in root.
