中国药学杂志
中國藥學雜誌
중국약학잡지
CHINESE PHARMACEUTICAL JOURNAL
2006年
17期
1342-1344
,共3页
乔春峰%韩全斌%宋景政%戴晋%莫世甫%徐宏喜
喬春峰%韓全斌%宋景政%戴晉%莫世甫%徐宏喜
교춘봉%한전빈%송경정%대진%막세보%서굉희
半枝莲%野黄芩苷%野黄芩素%木犀草素%芹菜素%高效液相色谱法
半枝蓮%野黃芩苷%野黃芩素%木犀草素%芹菜素%高效液相色譜法
반지련%야황금감%야황금소%목서초소%근채소%고효액상색보법
目的 建立测定半枝莲药材中野黄芩苷、野黄芩素、木犀草素和芹菜素含量的HPLC分析方法.方法 采用C18柱,测定野黄芩苷以甲醇-0.1%醋酸水溶液(40∶60)为流动相;测定野黄芩素、木犀草素和芹菜素以乙腈-0.1%醋酸水溶液为流动相,梯度洗脱(0→40min,乙腈15%→60%).流速为1.0 mL·min-1,检测波长为335 nm.结果 野黄芩苷、野黄芩素、木犀草素和芹菜素分别在0.012 4～0.992μg,0.006 675～0.667 5μg,0.004 84～0.484μg和0.006 875～0.687 5μg内呈良好的线性关系.所测10份半枝莲药材中,野黄芩苷、野黄芩素、木犀草素和芹菜素的含量范围分别为0.147%～0.592%,0.028%～0.296%,0.007%～0.084%和0.011%～0.217%.结论 本方法简便可行,适用于半枝莲药材中4种黄酮类成分的含量测定.
目的 建立測定半枝蓮藥材中野黃芩苷、野黃芩素、木犀草素和芹菜素含量的HPLC分析方法.方法 採用C18柱,測定野黃芩苷以甲醇-0.1%醋痠水溶液(40∶60)為流動相;測定野黃芩素、木犀草素和芹菜素以乙腈-0.1%醋痠水溶液為流動相,梯度洗脫(0→40min,乙腈15%→60%).流速為1.0 mL·min-1,檢測波長為335 nm.結果 野黃芩苷、野黃芩素、木犀草素和芹菜素分彆在0.012 4～0.992μg,0.006 675～0.667 5μg,0.004 84～0.484μg和0.006 875～0.687 5μg內呈良好的線性關繫.所測10份半枝蓮藥材中,野黃芩苷、野黃芩素、木犀草素和芹菜素的含量範圍分彆為0.147%～0.592%,0.028%～0.296%,0.007%～0.084%和0.011%～0.217%.結論 本方法簡便可行,適用于半枝蓮藥材中4種黃酮類成分的含量測定.
목적 건립측정반지련약재중야황금감、야황금소、목서초소화근채소함량적HPLC분석방법.방법 채용C18주,측정야황금감이갑순-0.1%작산수용액(40∶60)위류동상;측정야황금소、목서초소화근채소이을정-0.1%작산수용액위류동상,제도세탈(0→40min,을정15%→60%).류속위1.0 mL·min-1,검측파장위335 nm.결과 야황금감、야황금소、목서초소화근채소분별재0.012 4～0.992μg,0.006 675～0.667 5μg,0.004 84～0.484μg화0.006 875～0.687 5μg내정량호적선성관계.소측10빈반지련약재중,야황금감、야황금소、목서초소화근채소적함량범위분별위0.147%～0.592%,0.028%～0.296%,0.007%～0.084%화0.011%～0.217%.결론 본방법간편가행,괄용우반지련약재중4충황동류성분적함량측정.
