CAJ | 학술논문

背景:以往曾采用猴、犬、猪、兔等大动物作为腭中缝牵张动物模型,但存在费用高,样本量小,检测抗体难获得等缺点.Wistar大鼠头部较宽阔,便于腔方面的操作,且成本低,繁殖率高,作为腭中缝牵张模型,有可能克服以上缺陷.目的:建立大鼠腭中缝牵张实验动物模型,为进一步进行腭中缝牵张的实验动物研究提供基础. 方法:5周龄Wistar雄性大鼠20只,平均体质量65 g,随机分成实验组与对照组,每组10只.实验组采用0.014澳丝弯制双眼圈簧样扩弓装置,插入第一、二磨牙牙间隙,用光固化树脂粘结在大鼠磨牙舌侧进行固位;对照组安装未加力的同型扩弓装置.主动加力1周.在扩弓结束后分别对大鼠腭中缝进行X射线片,组织学观察.结果与结论:上颌骨X射线片发现实验组腭中缝明显扩宽,磨牙明显颊向倾斜.经光镜观察发现,实验组腭中缝偏口腔侧明显增宽,间充质细胞呈梭性,与牵张力方向一致.在其下方出现创伤性炎症反应,有明显的出血区.结果证实,实验大鼠腭中缝牵张动物模型满足实验研究的需要,具有操作简单、设计科学、可重复性强的特点.
배경:이왕증채용후、견、저、토등대동물작위악중봉견장동물모형,단존재비용고,양본량소,검측항체난획득등결점.Wistar대서두부교관활,편우강방면적조작,차성본저,번식솔고,작위악중봉견장모형,유가능극복이상결함.목적:건립대서악중봉견장실험동물모형,위진일보진행악중봉견장적실험동물연구제공기출. 방법:5주령Wistar웅성대서20지,평균체질량65 g,수궤분성실험조여대조조,매조10지.실험조채용0.014오사만제쌍안권황양확궁장치,삽입제일、이마아아간극,용광고화수지점결재대서마아설측진행고위;대조조안장미가력적동형확궁장치.주동가력1주.재확궁결속후분별대대서악중봉진행X사선편,조직학관찰.결과여결론:상합골X사선편발현실험조악중봉명현확관,마아명현협향경사.경광경관찰발현,실험조악중봉편구강측명현증관,간충질세포정사성,여견장력방향일치.재기하방출현창상성염증반응,유명현적출혈구.결과증실,실험대서악중봉견장동물모형만족실험연구적수요,구유조작간단、설계과학、가중복성강적특점.
BACKGROUND: Monkeys, dogs, pigs, rabbits and other large animals have bean applied previously to prepare animal models of midpelatel suture expansion, but there are high cost, small sample size, difficult to obtain antibodies and other disadvantages, Wistar rats have wide heads to facilitate cavity operation, with low cost and high reproduction rata, as the midpalatal suture model, it is possible to overcome the above deficiencies. OBJECTIVE: To establish a rat model of midpalatal suture expansion, and to supply basement for further relative researches of animal models. METHODS: Twenty Wistar male rats of 5 weeks old, average weight of 65 g, were randomly divided into 2 groups, a experiment and a control, with 10 rats in each group. Rats in experimental group were placed on the expansion appliance, inserting into the diastema between the first and second molars, then stick to molar lingual using light-cured resin for retention. The rats in control group were sham operated, followed by one weak of active expansion. After expansion, the midpalatal sections were observed by X-ray and light microscopy. RESULTS AND CONCLUSION: Maxillary X-ray film showed that midpalatal suture in experimental group was significantly widened, molar lead to cheek. Observed by light microscopy, partial oral side of midpalatal suture in the experimental group was obviously enlarged, mesenchymst ceils were spindle, in the same direction to tension force, Below it, traumatic inflammatory response appeared, with a clear bleeding area. The midpalatal suture expansion model in rats is available, simple and reprodudble.