中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
1期
48-52
,共5页
田志华%梁云燕%李振甫%王代树
田誌華%樑雲燕%李振甫%王代樹
전지화%량운연%리진보%왕대수
紫龙金%人乳腺癌细胞系MCF-7%增殖%凋亡
紫龍金%人乳腺癌細胞繫MCF-7%增殖%凋亡
자룡금%인유선암세포계MCF-7%증식%조망
Zilongjin%human breast cancer cell line MCF-7%proliferation%apoptosis
目的 观察复方中药紫龙金对人乳腺癌细胞系MCF-7增殖的抑制作用及对其凋亡的诱导作用,探讨紫龙金治疗乳腺癌的作用机制.方法 依据培养液紫龙金浓度的差异,实验共设4组:(1)对照组:不加紫龙金干预的1640培养液;(2)紫龙金低浓度组:1.5 mg生药/mL紫龙金培养液;(3)紫龙金中浓度组:3 mg生药/mL紫龙金培养液;(4)紫龙金高浓度组:6 mg生药/mL紫龙金培养液.采用细胞计数法绘制生长曲线及四甲基偶氮唑蓝(MTT)比色法检测不同浓度紫龙金对MCF-7细胞增殖能力的影响,并应用流式细胞术、Hoechst 33342核染色法及DNA梯度形成法测定紫龙金诱导凋亡的作用.结果 (1)与对照组比较,紫龙金各剂量组的细胞计数在各时间点(24、48、72、96、120、144 h)明显减少,其差异均有统计学意义(P<0.05);紫龙金各剂量组间的生长抑制率差异除低、中浓度组间在24、72 h无统计学意义外(P>0.05),各剂量组间两两比较在不同时间点(24、48、72、96、120、144 h),其差异均有统计学意义(P<0.05).紫龙金对MCF-7细胞的增殖抑制作用呈时间依赖性和剂量依赖性;(2)紫龙金使细胞阻滞在G_0/G_1期;(3)中、高浓度紫龙金诱导MCF-7细胞凋亡,并形成DNA ladder.结论 紫龙金能抑制人乳腺癌细胞MCF-7细胞增殖,并诱导细胞凋亡,从而起到抗肿瘤的作用.
目的 觀察複方中藥紫龍金對人乳腺癌細胞繫MCF-7增殖的抑製作用及對其凋亡的誘導作用,探討紫龍金治療乳腺癌的作用機製.方法 依據培養液紫龍金濃度的差異,實驗共設4組:(1)對照組:不加紫龍金榦預的1640培養液;(2)紫龍金低濃度組:1.5 mg生藥/mL紫龍金培養液;(3)紫龍金中濃度組:3 mg生藥/mL紫龍金培養液;(4)紫龍金高濃度組:6 mg生藥/mL紫龍金培養液.採用細胞計數法繪製生長麯線及四甲基偶氮唑藍(MTT)比色法檢測不同濃度紫龍金對MCF-7細胞增殖能力的影響,併應用流式細胞術、Hoechst 33342覈染色法及DNA梯度形成法測定紫龍金誘導凋亡的作用.結果 (1)與對照組比較,紫龍金各劑量組的細胞計數在各時間點(24、48、72、96、120、144 h)明顯減少,其差異均有統計學意義(P<0.05);紫龍金各劑量組間的生長抑製率差異除低、中濃度組間在24、72 h無統計學意義外(P>0.05),各劑量組間兩兩比較在不同時間點(24、48、72、96、120、144 h),其差異均有統計學意義(P<0.05).紫龍金對MCF-7細胞的增殖抑製作用呈時間依賴性和劑量依賴性;(2)紫龍金使細胞阻滯在G_0/G_1期;(3)中、高濃度紫龍金誘導MCF-7細胞凋亡,併形成DNA ladder.結論 紫龍金能抑製人乳腺癌細胞MCF-7細胞增殖,併誘導細胞凋亡,從而起到抗腫瘤的作用.
목적 관찰복방중약자룡금대인유선암세포계MCF-7증식적억제작용급대기조망적유도작용,탐토자룡금치료유선암적작용궤제.방법 의거배양액자룡금농도적차이,실험공설4조:(1)대조조:불가자룡금간예적1640배양액;(2)자룡금저농도조:1.5 mg생약/mL자룡금배양액;(3)자룡금중농도조:3 mg생약/mL자룡금배양액;(4)자룡금고농도조:6 mg생약/mL자룡금배양액.채용세포계수법회제생장곡선급사갑기우담서람(MTT)비색법검측불동농도자룡금대MCF-7세포증식능력적영향,병응용류식세포술、Hoechst 33342핵염색법급DNA제도형성법측정자룡금유도조망적작용.결과 (1)여대조조비교,자룡금각제량조적세포계수재각시간점(24、48、72、96、120、144 h)명현감소,기차이균유통계학의의(P<0.05);자룡금각제량조간적생장억제솔차이제저、중농도조간재24、72 h무통계학의의외(P>0.05),각제량조간량량비교재불동시간점(24、48、72、96、120、144 h),기차이균유통계학의의(P<0.05).자룡금대MCF-7세포적증식억제작용정시간의뢰성화제량의뢰성;(2)자룡금사세포조체재G_0/G_1기;(3)중、고농도자룡금유도MCF-7세포조망,병형성DNA ladder.결론 자룡금능억제인유선암세포MCF-7세포증식,병유도세포조망,종이기도항종류적작용.
Objective To study the effect of Zilongjin (ZLJ, a composite Chinese drug) on proliferation and apoptosis of human breast carcinoma cell line MCF-7.Methods MCF-7 cells were randomly divided into four groups depending on the culture solution used, the control group, cultured with 1640 medium not contained ZLJ; and the three ZJL groups cultured with medium contained low (1.5 mg/mL), moderate (3 mg/mL) and high (6 mg/mL) dosage of ZLJ crude drug respectively. The changes of cell proliferation were assessed by cell growth curve assay and methyl thiazolyl tetrazolium (MTT) assay. And the cell apoptosis was analyzed by flow cytometry, Hoechst 33342 staining and DNA ladder assay. Results Compared with that in the normal control, the counts of cells in the three ZLJ groups were decreased significantly (P< 0.05) at such time point as 24, 48, 72, 96, 120, and 144 h. Furthermore, apart from the comparation of the growth inhibition rate between the low and moderate dosage group at 24 and 72 h which were found to be no significant difference (P> 0.05), the comparations of that among the three ZLJ groups appeared to be significant difference (P< 0.05). The inhibitory effect of ZLJ on cell proliferation of MCF-7 was time-and dose-dependent; it could retard cells in G0/G1 cell phase; apoptosis of MCF-7 cell was induced by modrate and high dosage of ZLJ with revealing of apoptotic body and DNA ladder formation. Conclusion ZLJ shows cell proliferation inhibitory and apoptosis inducing effects on human breast cancer cell line MCF-7, and thus to realize its anti-tumor action.
