CAJ | 학술논문

目的探究高糖以及SB203580对大鼠肾系膜细胞的色素上皮细胞衍生因子(PEDF)表达的影响.方法将大鼠HBZY-1肾小球系膜细胞分为7组,将高糖作为刺激因子,SB203580作为干预因素.分别设正常对照组、高糖组(包括3种浓度的葡萄糖)、甘露醇组、治疗组以及溶剂对照组.用Real time Quantitative PCR法测定各组系膜细胞PEDF mRNA表达.结果 (1)正常组系膜细胞PEDF在mRNA的水平上有表达,高糖组系膜细胞PEDF mRNA的表达较正常对照组明显降低.(2)治疗组的系膜细胞PEDF mRNA表达较高糖组明显增加.结论高糖可抑制大鼠肾系膜细胞PEDF的表达,并与P38MAPK信号通路有关.
목적 탐구고당이급SB203580대대서신계막세포적색소상피세포연생인자(PEDF)표체적영향.방법 장대서HBZY-1신소구계막세포분위7조,장고당작위자격인자,SB203580작위간예인소.분별설정상대조조、고당조(포괄3충농도적포도당)、감로순조、치료조이급용제대조조.용Real time Quantitative PCR법측정각조계막세포PEDF mRNA표체.결과 (1)정상조계막세포PEDF재mRNA적수평상유표체,고당조계막세포PEDF mRNA적표체교정상대조조명현강저.(2)치료조적계막세포PEDF mRNA표체교고당조명현증가.결론 고당가억제대서신계막세포PEDF적표체,병여P38MAPK신호통로유관.
Objective To explore the effect of high glucose and SB203580 on the expression of pigment epithelium-derived factor(PEDF)in cultured glomerular mesangial cells(GMC).Methods Cultured HBZY-1 rat GMC were divided into 7 groups(glucose was used as a stimulating factor,SB203580 as an intervenor):normol glucose(5.6mmol/L)group,high glucose-treated group(10,20,30mmol/L),mannitol-treated group,therapy(SB203580)group,drug-solvent control group.We measured the expression of PEDF of each glomerular mesangial cells group by real time quantitative PCR.Results 1.Normol glucose group showed the higher expression of PEDF than did the high glucose group.The expression of PEDF mRNA of group of normol glucose was 5.3,11.3,27.9 times elevated above high glucose groups(all P<0.05).2.The expression of PEDF mRNA was higher in therapy group than in high glucose group.The expression of PEDF mRNA in therapy group was 31.8 times that of high glucose group(P<0.05).Conclusions High glucose can decrease significantly the expression of PEDF in cultured mesangial cells,which is independent of the osmotic pressure.Downregulated expression of PEDF induced by high glucose is related with P38MAPK signal transduction pathways