复旦学报（医学版）
複旦學報（醫學版）
복단학보（의학판）
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2007年
6期
800-805,820
,共7页
胡凯莉%谢月玲%陆伟%蒋新国
鬍凱莉%謝月玲%陸偉%蔣新國
호개리%사월령%륙위%장신국
复乳溶剂挥发法%NC-1900%MePEG-PLA%纳米粒
複乳溶劑揮髮法%NC-1900%MePEG-PLA%納米粒
복유용제휘발법%NC-1900%MePEG-PLA%납미립
double emulsion/solvent evaporation method%arginine-vasopressin fragment analog (NC-1900)%MePEG-PLA%nanoparticle
目的 制备载NC-1900的MePEG-PLA纳米粒.方法 以溶液聚合法合成不同分子量的MePEG-PLA聚合物为材料,采用复乳溶剂挥发法制备纳米粒,以纳米粒粒径和NC-1900包封率为考察指标,设计正交试验及多元回归分析优化处方与工艺,并对纳米粒进行表征,结合体外泄漏试验筛选出最优的NC-1900纳米粒载体.结果 以MePEG3000-PLA44800为材料根据最优处方制得的载NC-1900 NPs均匀圆整,平均粒径为(77 ± 11) nm,包封率约为( 21.40 ±0.10 ) % ,在pH 7.4的 PBS溶液和空白血浆中48 h NC-1900泄漏率分别小于5% 和15%.结论 最优处方制得的MePEG3000-PLA44800纳米粒适宜作为NC-1900的载体.
目的 製備載NC-1900的MePEG-PLA納米粒.方法 以溶液聚閤法閤成不同分子量的MePEG-PLA聚閤物為材料,採用複乳溶劑揮髮法製備納米粒,以納米粒粒徑和NC-1900包封率為攷察指標,設計正交試驗及多元迴歸分析優化處方與工藝,併對納米粒進行錶徵,結閤體外洩漏試驗篩選齣最優的NC-1900納米粒載體.結果 以MePEG3000-PLA44800為材料根據最優處方製得的載NC-1900 NPs均勻圓整,平均粒徑為(77 ± 11) nm,包封率約為( 21.40 ±0.10 ) % ,在pH 7.4的 PBS溶液和空白血漿中48 h NC-1900洩漏率分彆小于5% 和15%.結論 最優處方製得的MePEG3000-PLA44800納米粒適宜作為NC-1900的載體.
목적 제비재NC-1900적MePEG-PLA납미립.방법 이용액취합법합성불동분자량적MePEG-PLA취합물위재료,채용복유용제휘발법제비납미립,이납미립립경화NC-1900포봉솔위고찰지표,설계정교시험급다원회귀분석우화처방여공예,병대납미립진행표정,결합체외설루시험사선출최우적NC-1900납미립재체.결과 이MePEG3000-PLA44800위재료근거최우처방제득적재NC-1900 NPs균균원정,평균립경위(77 ± 11) nm,포봉솔약위( 21.40 ±0.10 ) % ,재pH 7.4적 PBS용액화공백혈장중48 h NC-1900설루솔분별소우5% 화15%.결론 최우처방제득적MePEG3000-PLA44800납미립괄의작위NC-1900적재체.
Purpose To optimize the preparation of NC-1900 loaded MePEG-PLA nanoparticles (NPs). Methods MePEG-PLA copolymers of different molecular weight synthesized by solvent poly-merization method were used to prepare NC-1900 loaded MePEG-PLA NPs by double emulsion/solvent evaporation method.Orthogonal experimental design and multiple regressions were used to optimize the preparation method with nanoparticle size and NC-1900 encapsulation efficiency (EE) as res-ponse variables.NPs were characterized by particle size and Zeta potential detector and transmission electron microscope.The leakage of NC-1900 from NPs was evaluated by high-performance liquid chromatography (HPLC) detection. Results MePEG3000-PLA44800 NPs prepared according to the optimized conditions had a mean diameter of (77 ± 11) nm and EE of (21.4 ± 0.1) %.Only 5% and 15% of NC-1900 were leaked in pH 7.4 PBS and blank plasma at the end of 48 h,respectively. Conc-lusions The optimized MePEG3000-PLA44800 NPs is a favorable carrier for NC-1900.
