CAJ | 학술논문

本试验旨在研究硒酵母对西门塔尔牛营养物质消化代谢和血清生化指标的影响.选用16头平均体重420kg,年龄2.5岁的西门塔尔牛阉牛,采用随机区组设计分为4组,以硒酵母为硒源,分别在日粮中添加硒0、0.3、0.6和0.9 mg/kg.结果表明:日粮养分表观消化率、矿物元素表观存留率、消化氮和沉积氮/消化氮比例均以0.3 mg/kg与0.6 mg/kg组较高(P＜0.05);0.6 mg/kg组甘油三酯显著提高,处理组血糖显著增加,0.6 mg/kg和0.9 mg/kg组白蛋白增加(P＜0.05),总胆固醇、总蛋白和尿素氮差异不显著(P＞0.05);0.6 mg/kg和0.9 mg/kg组血清GOT和GPT含量显著提高(P＜0.05),AKP、ACP和LDH差异均不显著(P＞0.05);处理组GSH-px活性显著提高(P＜0.05),以0.6 mg/kg组最高;0.6 mg/kg和0.9 mg/kg组MDA显著降低(P＜0.05);0.6 mg/kg组GSH含量显著增加(P＜0.05);0.6 mg/kg和0.9 mg/kg组血清硒含量显著增加(P＜0.05).日粮以硒酵母为硒源时,添加硒0.6 mg/kg显著促进营养物质消化代谢和提高机体抗氧化能力,建议日粮加硒为0.6 mg/kg.
본시험지재연구서효모대서문탑이우영양물질소화대사화혈청생화지표적영향.선용16두평균체중420kg,년령2.5세적서문탑이우엄우,채용수궤구조설계분위4조,이서효모위서원,분별재일량중첨가서0、0.3、0.6화0.9 mg/kg.결과표명:일량양분표관소화솔、광물원소표관존류솔、소화담화침적담/소화담비례균이0.3 mg/kg여0.6 mg/kg조교고(P＜0.05);0.6 mg/kg조감유삼지현저제고,처리조혈당현저증가,0.6 mg/kg화0.9 mg/kg조백단백증가(P＜0.05),총담고순、총단백화뇨소담차이불현저(P＞0.05);0.6 mg/kg화0.9 mg/kg조혈청GOT화GPT함량현저제고(P＜0.05),AKP、ACP화LDH차이균불현저(P＞0.05);처리조GSH-px활성현저제고(P＜0.05),이0.6 mg/kg조최고;0.6 mg/kg화0.9 mg/kg조MDA현저강저(P＜0.05);0.6 mg/kg조GSH함량현저증가(P＜0.05);0.6 mg/kg화0.9 mg/kg조혈청서함량현저증가(P＜0.05).일량이서효모위서원시,첨가서0.6 mg/kg현저촉진영양물질소화대사화제고궤체항양화능력,건의일량가서위0.6 mg/kg.
To evaluate the effects of Se-yeast on nutrients digestibility, nitrogen balance and serum biochemical parameters, sixteen Simmental steers consuming a corn straw diet were stratified by weight and randomly assigned in a block design to treatments. Treatments were consisted of 0 (control), 0.3, 0.6 or 0.9 mg of supplemental Se (as Se-yeast) per kg diet DM. The results showed that the digestibility of nutrients, the apparent deposit rate of mineral, digestion nitrogen and the utilization of nitrogen in Treatment 1 and Treatment 2 supplemented 0.3 and 0.6 mg Se were higher than other Treatments (P＜ 0.05). Serum triglycerides concentration in Treatment 2 supplemented 0.6 mg Se was higher than other Treatments and the control (P＜0.05). Serum glucose concentrations in all Treatments supplemented Se increased significantly (P＜0.05). Serum albumin concentrations in Treatment 2 and Treatment 3 supplemented 0.6 and 0.9 mg Se increased (P＜0.05). Total serum cholesterol, total protein and urine nitrogen concentrations were not affected. The activities of serum GOT and GPT in Treatment 2 and Treatment 3 supplemented 0.6 mg and 0.9 mg Se were higher than other Treatments (P＜0.05). Serum AKP, ACP and LDH activities were not affected (P＞0. 05). Serum GSH-px activity in Treatment 2 supplemented 0.6 mg Se increased (P＜0.05). Serum GSH concentration in Treatment 2 supplemented 0.6 mg Se increased (P＜0.05). Serum MDA and Se concentration in Treatment 2 and Treatment 3supplemented 0.6 mg and 0.9 mg Se decreased significantly (P＜0.05). These data indicated that the digestion and utilization of nutrients were improved by Treatment 1 and Treatment 2 0.3～0.6 mg Se per kg diet DM. The activities of serum enzyme increased and MDA concentration decreased by Treatment 2 supplemented 0.6 mg Se per kg diet DM. The optimum Se supplemented dose is 0.6 mg Se per kg diet DM in this experiment.