背景:有实验证明将碱性成纤维细胞生长因子和鸦胆子乳膏联合应用能促进创伤愈合并抑制瘢痕形成.目的:观察碱性成纤维细胞生长因子与鸦胆子油乳剂联用对兔兔背部刀割伤的促愈合作用.设计:随机对照观察.单位:暨南大学医药生物技术研究开发中心,暨南大学药学院.材料:实验于2005-06/09在暨南大学药学院完成.选用8只北京产大耳白兔,雌雄各半,体质量2.0～2.5 kg,由南方医科大学实验动物中心提供.碱性成纤维细胞生长因子无菌冻干粉制剂(广州长生基因药业有限公司,批号:20040219),临用前用注射用水配制成溶液.鸦胆子乳剂由沈阳药科大学药剂研究室姚崇舜教授提供(浙江三九邦而康药业有限公司,批号:20040206).方法:将实验兔麻醉消毒,背中部脊柱两侧各旁开1.5 cm处,由前至后共切割5个圆形创面,直径1.8 cm,面积2.54 cm2,抽签法随机将每只实验兔的5个创面分为碱性成纤维细胞生长因子治疗组(碱性成纤维细胞生长因子用量为90 U/cm2)、综合治疗组(碱性成纤维细胞生长因子用量为90 U/cm2,30 min后涂抹鸦胆子乳膏30 mg/cm2)、鸦胆子乳治疗组(涂抹鸦胆子乳膏30 mg/cm2)、空白基质组(空白基质30 mg/cm2)及空白对照组(涂抹生理盐水).伤后即开始用药,以后每天换药1次,至伤后第16天.术后用透明膜描记法(用洁净保鲜膜覆盖伤口,描出创面大小,剪下称重,换算成面积计算)记录伤后第4,8,12,16天创面面积;用注水法测量伤腔容积;伤后第8,16天取创面组织,经常规组织切片染色后观察创面肉芽组织生长与再上皮化情况.主要观察指标:各组实验兔伤后不同时间点创面面积、伤腔容积及创面肉芽组织生长与再上皮化情况.结果:纳入实验兔8只全部进入结果分析.①各组实验兔不同时间点创面面积:综合治疗组用药后4,8,12 d创面面积分别为(2.05±0.35),(1.59±0.25),(0.55±0.25)cm2,小于空白对照组相应时间点[(2.53±0.30),(2.41±0.19),(1.09±0.34)cm2,P＜0.05～0.01].碱性成纤维细胞生长因子治疗组用药后8,12 d创面面积分别为(1.71±0.31),(0.51±0.10)cm2,小于空白对照组相应时间点(P＜0.05～0.01).②各组实验兔伤腔容积:碱性成纤维细胞生长因子治疗组实验兔伤后4 d创面体积为(0.49±0.12)mL,小于空白对照组相应时间点[(0 59±0.1)mL,P＜0.05],综合治疗组伤后4,8 d创面体积分别为(0.47±0.12),(0.30±0.08)mL,小于空白对照组相应时间点[(0.59±0.1),(0.41±0.07)mL,P＜0 05,0.01].③各组实验兔创面组织肉芽组织生长与再上皮化情况:综合治疗组伤后8 d显示炎症反应较轻,成纤维细胞增生显著,其毛细血管胚芽与成纤维细胞数量多于空白对照组;模型组与空白基质组情况基本相同,炎症反应重,肉芽组织增加不明显,新生毛细血管少,表皮细胞增殖不明显.伤后16天,综合治疗组的创面收缩与再上皮化明显,新生上皮向创面中心爬行较快.结论:鸦胆子乳剂及碱性成纤维细胞生长因子联合应用对兔背部刀伤创面有明显的促修复作用.
揹景:有實驗證明將堿性成纖維細胞生長因子和鴉膽子乳膏聯閤應用能促進創傷愈閤併抑製瘢痕形成.目的:觀察堿性成纖維細胞生長因子與鴉膽子油乳劑聯用對兔兔揹部刀割傷的促愈閤作用.設計:隨機對照觀察.單位:暨南大學醫藥生物技術研究開髮中心,暨南大學藥學院.材料:實驗于2005-06/09在暨南大學藥學院完成.選用8隻北京產大耳白兔,雌雄各半,體質量2.0～2.5 kg,由南方醫科大學實驗動物中心提供.堿性成纖維細胞生長因子無菌凍榦粉製劑(廣州長生基因藥業有限公司,批號:20040219),臨用前用註射用水配製成溶液.鴉膽子乳劑由瀋暘藥科大學藥劑研究室姚崇舜教授提供(浙江三九邦而康藥業有限公司,批號:20040206).方法:將實驗兔痳醉消毒,揹中部脊柱兩側各徬開1.5 cm處,由前至後共切割5箇圓形創麵,直徑1.8 cm,麵積2.54 cm2,抽籤法隨機將每隻實驗兔的5箇創麵分為堿性成纖維細胞生長因子治療組(堿性成纖維細胞生長因子用量為90 U/cm2)、綜閤治療組(堿性成纖維細胞生長因子用量為90 U/cm2,30 min後塗抹鴉膽子乳膏30 mg/cm2)、鴉膽子乳治療組(塗抹鴉膽子乳膏30 mg/cm2)、空白基質組(空白基質30 mg/cm2)及空白對照組(塗抹生理鹽水).傷後即開始用藥,以後每天換藥1次,至傷後第16天.術後用透明膜描記法(用潔淨保鮮膜覆蓋傷口,描齣創麵大小,剪下稱重,換算成麵積計算)記錄傷後第4,8,12,16天創麵麵積;用註水法測量傷腔容積;傷後第8,16天取創麵組織,經常規組織切片染色後觀察創麵肉芽組織生長與再上皮化情況.主要觀察指標:各組實驗兔傷後不同時間點創麵麵積、傷腔容積及創麵肉芽組織生長與再上皮化情況.結果:納入實驗兔8隻全部進入結果分析.①各組實驗兔不同時間點創麵麵積:綜閤治療組用藥後4,8,12 d創麵麵積分彆為(2.05±0.35),(1.59±0.25),(0.55±0.25)cm2,小于空白對照組相應時間點[(2.53±0.30),(2.41±0.19),(1.09±0.34)cm2,P＜0.05～0.01].堿性成纖維細胞生長因子治療組用藥後8,12 d創麵麵積分彆為(1.71±0.31),(0.51±0.10)cm2,小于空白對照組相應時間點(P＜0.05～0.01).②各組實驗兔傷腔容積:堿性成纖維細胞生長因子治療組實驗兔傷後4 d創麵體積為(0.49±0.12)mL,小于空白對照組相應時間點[(0 59±0.1)mL,P＜0.05],綜閤治療組傷後4,8 d創麵體積分彆為(0.47±0.12),(0.30±0.08)mL,小于空白對照組相應時間點[(0.59±0.1),(0.41±0.07)mL,P＜0 05,0.01].③各組實驗兔創麵組織肉芽組織生長與再上皮化情況:綜閤治療組傷後8 d顯示炎癥反應較輕,成纖維細胞增生顯著,其毛細血管胚芽與成纖維細胞數量多于空白對照組;模型組與空白基質組情況基本相同,炎癥反應重,肉芽組織增加不明顯,新生毛細血管少,錶皮細胞增殖不明顯.傷後16天,綜閤治療組的創麵收縮與再上皮化明顯,新生上皮嚮創麵中心爬行較快.結論:鴉膽子乳劑及堿性成纖維細胞生長因子聯閤應用對兔揹部刀傷創麵有明顯的促脩複作用.
배경:유실험증명장감성성섬유세포생장인자화아담자유고연합응용능촉진창상유합병억제반흔형성.목적:관찰감성성섬유세포생장인자여아담자유유제련용대토토배부도할상적촉유합작용.설계:수궤대조관찰.단위:기남대학의약생물기술연구개발중심,기남대학약학원.재료:실험우2005-06/09재기남대학약학원완성.선용8지북경산대이백토,자웅각반,체질량2.0～2.5 kg,유남방의과대학실험동물중심제공.감성성섬유세포생장인자무균동간분제제(엄주장생기인약업유한공사,비호:20040219),림용전용주사용수배제성용액.아담자유제유침양약과대학약제연구실요숭순교수제공(절강삼구방이강약업유한공사,비호:20040206).방법:장실험토마취소독,배중부척주량측각방개1.5 cm처,유전지후공절할5개원형창면,직경1.8 cm,면적2.54 cm2,추첨법수궤장매지실험토적5개창면분위감성성섬유세포생장인자치료조(감성성섬유세포생장인자용량위90 U/cm2)、종합치료조(감성성섬유세포생장인자용량위90 U/cm2,30 min후도말아담자유고30 mg/cm2)、아담자유치료조(도말아담자유고30 mg/cm2)、공백기질조(공백기질30 mg/cm2)급공백대조조(도말생리염수).상후즉개시용약,이후매천환약1차,지상후제16천.술후용투명막묘기법(용길정보선막복개상구,묘출창면대소,전하칭중,환산성면적계산)기록상후제4,8,12,16천창면면적;용주수법측량상강용적;상후제8,16천취창면조직,경상규조직절편염색후관찰창면육아조직생장여재상피화정황.주요관찰지표:각조실험토상후불동시간점창면면적、상강용적급창면육아조직생장여재상피화정황.결과:납입실험토8지전부진입결과분석.①각조실험토불동시간점창면면적:종합치료조용약후4,8,12 d창면면적분별위(2.05±0.35),(1.59±0.25),(0.55±0.25)cm2,소우공백대조조상응시간점[(2.53±0.30),(2.41±0.19),(1.09±0.34)cm2,P＜0.05～0.01].감성성섬유세포생장인자치료조용약후8,12 d창면면적분별위(1.71±0.31),(0.51±0.10)cm2,소우공백대조조상응시간점(P＜0.05～0.01).②각조실험토상강용적:감성성섬유세포생장인자치료조실험토상후4 d창면체적위(0.49±0.12)mL,소우공백대조조상응시간점[(0 59±0.1)mL,P＜0.05],종합치료조상후4,8 d창면체적분별위(0.47±0.12),(0.30±0.08)mL,소우공백대조조상응시간점[(0.59±0.1),(0.41±0.07)mL,P＜0 05,0.01].③각조실험토창면조직육아조직생장여재상피화정황:종합치료조상후8 d현시염증반응교경,성섬유세포증생현저,기모세혈관배아여성섬유세포수량다우공백대조조;모형조여공백기질조정황기본상동,염증반응중,육아조직증가불명현,신생모세혈관소,표피세포증식불명현.상후16천,종합치료조적창면수축여재상피화명현,신생상피향창면중심파행교쾌.결론:아담자유제급감성성섬유세포생장인자연합응용대토배부도상창면유명현적촉수복작용.
BACKGROUND: It has been proved that the application of basic fibroblast growth factor (bFGF) combined with Brucea Javanica oil emulsion can accelerate wound healing and inhibit scar formation.OBJECTIVE: To observe the effects of bFGF plus Brucea Javanica oil emulsion cream on accelerating the skin wound healing of rabbits.DESIGN: A randomized controlled observation.SETTING: Center of Biotechnological Research and Development, Jinan University; College of Pharmacy, Jinan University.MATERIALS:The experiment was carried out in the College of Pharmacy, Jinan University from June to September in 2004. Eight Beijing big-ear white rabbits (4 males and 4 females) of 2.0-2.5 kg were provided by the experimental animal center of Southern Medical University (certification number: SoKx-2002-010). bFGF sterile freeze dried powder agent,provided by Guangzhou Changsheng Gene Pharmaceutical Co.,Ltd (batch number: 20040219; specific activity was 6 000 U/bottle), was prepared to solution with water for injection before application. Brucea Javanica oil emulsion (manufactured by Zhejiang 999 Bang'erkang Pharmaceutical Co.,Ltd.) was provided by Professor Yao from staff Room of Pharmacy, Shenyang Pharmaceutical University.METHODS: The rabbits were anesthetized and disinfected, 5 round wounds with diameter of 1.8 cm and area of 2.54 cm2were induced from front to back by bilateral incision at 1.5 cm from middle spine of rabbit. The 5 wounds of each rabbit were randomly divided into bFGF-treated group (90 U/cm2), bFGF+Brucea Javanica oil emulsion group [the wound was smeared with Brucea Javanica oil emulsion (30 mg/cm2) 30 minutes after bFGF (90 U/cm2)], Brucea Javanica oil emulsion treated group [the wound was smeared with Brucea Javanica oil emulsion (30 mg/cm2)], blank emulsion group (30 mg/cm2) and blank control group (the wound was smeared with saline). The medication was give immediately after injury, and changed once a day for 16 days. At 4, 8, 12 and 16 days after injury, the wound areas were recorded with the method of hyaline membrane tracing (the wound was covered with clean saran wrap, the size of wound was traced,and then sheared to be weighed, and converted to calculate the area), and the volume of wounded cavity was measured by infusing water. At 8 and 16 days, the wound tissue was removed, stained after routine tissue sections, and the conditions of growth of granulation tissue and reepithelization on the wound surface were observed.MAIN OUTCOME MEASURE: The wound area, volume of wounded cavity, and the conditions of growth of granulation tissue and reepithelization on the wound surface were obviously at different time points after injury in each group.RESULTS:All the 8 rabbits were involved in the analysis of results. ① Wound areas at different time points in each group: The wound areas in the bFGF+Brucea Javanica oil emulsion group at 4, 8 and 12 days after medication were smaller than those in the blank control group at corresponding time points [(2.05±0.35), (1.59±0.25), (0.55±0.25) cm2;(2.53±0.30), (2.41±0.19), (1.09±0.34) cm2, P＜0.05-0.01]. The wound areas in the bFGF group at 8 and 12 days after medication were (1.71±0.31) and (0.51±0.10) cm2, which were significantly smaller than those in the blank control group at corresponding time points (P＜0.05-0.01). ② Volume of the wounded cavity in each group: The wound volume in the bFGF group at 4 days after injury was markedly smaller than that in the blank control group at corresponding time point [(0.49±0.12), (0.59±0.1) mL, P＜0.05]. The wound volumes in the bFGF+Brucea Javanica oil emulsion group at 4 and 8days after injury were significantly smaller than those in the blank control group at corresponding time points [(0.47±0.12), (0.30±0.08) mL; (0.59±0.1), (0.41±0.07) mL, P＜0.05, 0.01]. ③ Growth of granulation tissue and reepithelization on the wound surface in each group: At 8 days after injury, the inflammatory reaction was milder and fibroblasts proliferated significantly in the bFGF+Brucea Javanica oil emulsion group, and the numbers of capillary plumules and fibroblasts were significantly more than those in the blank control group. The conditions in the blank control group and blank emulsion group were generally the same that there were severe inflammatory reactions, obvious increase of granulation tissue, fewer new capillaries, and unobvious proliferation of epidermic cells. At 16 days after injury, the contraction and reepithelization on the wound surface were obvious, and the new epithelia went towards the wound center rapidly in the bFGF+Brucea Javanica oil emulsion group.CONCLUSION : The application of Brucea Javanica oil emulsion plus bFGF can obviously accelerate the repair of incised wound on the back of rabbits.
