CAJ | 학술논문

首次从长春花中克隆了Crlea(Crlea for Catharanthus roseus late embryogenesis abundant)的全长基因,采用荧光定量PCR方法对干旱胁迫下长春花叶片和根部Crlea基因的表达模式进行监测,结果表明,在0.5～8h的胁迫时间中,叶片和根部的Crlea基因表现出相似的积累模式.长春花Crlea基因的表达随着胁迫时间的延长而表达增强.在叶片中,在6 h和8 h的干旱处理后,Crlea基因表达显著提高,分别是未处理材料的9.984和20.431倍.在根部, 在8 h的处理后,Crlea基因的表达量显著提高(2.831倍于对照).初步结果表明Crlea基因的表达没有组织特异性,并且为干旱胁迫正调控.
수차종장춘화중극륭료Crlea(Crlea for Catharanthus roseus late embryogenesis abundant)적전장기인,채용형광정량PCR방법대간한협박하장춘화협편화근부Crlea기인적표체모식진행감측,결과표명,재0.5～8h적협박시간중,협편화근부적Crlea기인표현출상사적적루모식.장춘화Crlea기인적표체수착협박시간적연장이표체증강.재협편중,재6 h화8 h적간한처리후,Crlea기인표체현저제고,분별시미처리재료적9.984화20.431배.재근부, 재8 h적처리후,Crlea기인적표체량현저제고(2.831배우대조).초보결과표명Crlea기인적표체몰유조직특이성,병차위간한협박정조공.
A full-length Crlea(Crlea for Catharanthus roseus late embryogenesis abundant) gene was first isolated from Catharanthus roseus. Gene expression profiles of Crlea gene in leaves and roots under drought stress were monitored by real-time quantitative PCR. The results showed that a similar accumulation pattern of Crlea gene in leaves and roots over the observation period of 0.5 to 8 hours. The expression of Crlea mRNA was strengthened with the prolongation of stress time. In leaves, expression amounts of Crlea gene were 9.984 and 20.431 times higher than that of control respectively at 6 and 8 h. Similarly, the expression amounts of Crlea gene in root obviously increased (2.831 times higher than that of control) at 8 h. Primary results show the expression of Crlea gene is non-tissue-specific and up-regulated under drought stress.