CAJ | 학술논문

[目的]研究丙烯腈(AN)的类性激素样作用.[方法]①采用经典大鼠子宫增重方法测试丙烯腈的类雌激素样作用,对去卵巢SD大鼠皮下注射剂量分别为0、10、15、25、40、60 mg/kg受试物丙烯腈,每天一次、连续3天;同时皮下注射剂量为0.001 mg/kg雌二醇玉米油溶液为阳性对照组.最后一次给药24 h后,将大鼠麻醉后取血并处死,观察受试动物子宫湿重、干重、肝重、肾重等指标.②研究丙烯腈的抗雄激素作用.采用OECD推荐的内分泌干扰体内筛检实验方法(Hershberger试验),对雄性去势大鼠皮下注射剂量为0.4mg/kg体重丙酸睾酮,同时经口灌胃剂量分别为0,10,20,30,40,50 mg/kg体重的受试物丙烯腈以及25 mg/kg体重的阳性参照物氟他胺(flutamide,FLU),每天一次,连续10天.最后一次给药24 h后,将大鼠麻醉后取血并处死,观察受试动物前列腺、精囊腺、阴茎、肛提肌/球体海绵体肌、尿道球腺等附性腺组织(sex accessorytissue,SAT)重量和肝重、肾重等指标.[结果]①子宫增重实验:丙烯腈各剂量组大鼠子宫干重、湿重与溶剂对照组无显著差异(P＞0.05),阳性对照组与溶剂对照组及丙烯腈各剂量组比较,子宫干重、湿重差异存在显著性(P＜0.05).②Hershberger试验:丙烯腈各剂量组与阴性对照组相比,SAT重量差异无显著性(P＞0.05),阳性对照组(FLU)与阴性对照组相比,SAT重量差别具有显著性(P＜0.05).丙烯腈各剂量组肝、肾重量与阴性对照组差别无显著性(P＞0.05),提示受试动物无明显系统毒性.[结论]采用子宫增重测试和Hershberger体内筛检试验未发现丙烯腈具有类性激素作用.
[목적]연구병희정(AN)적류성격소양작용.[방법]①채용경전대서자궁증중방법측시병희정적류자격소양작용,대거란소SD대서피하주사제량분별위0、10、15、25、40、60 mg/kg수시물병희정,매천일차、련속3천;동시피하주사제량위0.001 mg/kg자이순옥미유용액위양성대조조.최후일차급약24 h후,장대서마취후취혈병처사,관찰수시동물자궁습중、간중、간중、신중등지표.②연구병희정적항웅격소작용.채용OECD추천적내분비간우체내사검실험방법(Hershberger시험),대웅성거세대서피하주사제량위0.4mg/kg체중병산고동,동시경구관위제량분별위0,10,20,30,40,50 mg/kg체중적수시물병희정이급25 mg/kg체중적양성삼조물불타알(flutamide,FLU),매천일차,련속10천.최후일차급약24 h후,장대서마취후취혈병처사,관찰수시동물전렬선、정낭선、음경、항제기/구체해면체기、뇨도구선등부성선조직(sex accessorytissue,SAT)중량화간중、신중등지표.[결과]①자궁증중실험:병희정각제량조대서자궁간중、습중여용제대조조무현저차이(P＞0.05),양성대조조여용제대조조급병희정각제량조비교,자궁간중、습중차이존재현저성(P＜0.05).②Hershberger시험:병희정각제량조여음성대조조상비,SAT중량차이무현저성(P＞0.05),양성대조조(FLU)여음성대조조상비,SAT중량차별구유현저성(P＜0.05).병희정각제량조간、신중량여음성대조조차별무현저성(P＞0.05),제시수시동물무명현계통독성.[결론]채용자궁증중측시화Hershberger체내사검시험미발현병희정구유류성격소작용.
[ Objective ] To assess the estrogenic and anti-androgenic effects of acrylonitrile (AN) by in-vivo uterotrophic and Hershberger assays proposed by the OECD. [ Methods ] For the uterotrophic assay, AN was injected subcutaneously at the doses of 0, 10, 15, 25, 40 and 60mg/kg to 56-day-old ovariectomized rats for 3 days. In some rats, 17β-estradiol (EE) was also injected subcutaneously at a dose of 0.001 mg/kg as a positive control group. For the Hershberger assay, AN was administered orally at doses of 0, 10, 20, 30, 40 and 50 mg/kg to 56-day-old castrated rats for 10 days. 0.4 mg/kg testosterone propionate (TP) was also administered by subcutaneous injection after the administration of AN. In some rats, flutamide (FLU) was also administered orally at a dose of 25 mg/kg as the reference chemical for anti-vandrogenicity. [ Results ] For the uterotrophic assay, there were essentially no differences in the uterine wet or blotted weights between the control and any of the AN-treated groups. For the Hershberger assay,the weights of the accessory sex organs of the castrated rats showed no significant differences between the oil plus TP-treated group and any of the AN plus TP-treated groups. [ Conclusion ] These findings suggest that AN dose not have any endocrine-disrupting properties in in-vivo screening tests.