CAJ | 학술논문

建立了毛细管电泳-紫外检测法测定硝酸咪康唑乳膏、小儿止咳糖浆及复方苦参水杨酸散中苯甲酸和山梨酸钾的方法.在230 nm波长处以焦性没食子酸为内标物,分离电压为20 kV,分离温度为25℃,用20 mmol/L硼砂缓冲液(pH 9.2)作毛细管电泳的运行液,被测组分与内标物得到快速分离.苯甲酸和山梨酸钾的进样质量浓度在1～400 mg/L范围内与电泳峰面积呈良好的线性关系,相关系数r均为0.9999,检出限均为0.15 mg/L.方法可用于药品中苯甲酸和山梨酸钾含量的测定.
건립료모세관전영-자외검측법측정초산미강서유고、소인지해당장급복방고삼수양산산중분갑산화산리산갑적방법.재230 nm파장처이초성몰식자산위내표물,분리전압위20 kV,분리온도위25℃,용20 mmol/L붕사완충액(pH 9.2)작모세관전영적운행액,피측조분여내표물득도쾌속분리.분갑산화산리산갑적진양질량농도재1～400 mg/L범위내여전영봉면적정량호적선성관계,상관계수r균위0.9999,검출한균위0.15 mg/L.방법가용우약품중분갑산화산리산갑함량적측정.
A method was developed for the determination of benzoic acid and potassium sorbate in miconazole nitrate cream, syrup urgent support and compound flavescent sophora and salicylic acid powder by capillary electrophoresis. At wavelength of 230 nm, using pyrogallic acid as the internal standard, the samples were analyzed with 20 mmol/L sodium borate buffer (pH 9.2) as running buffer at a capillary temperature of 25 ℃ and a voltage of 20 kV. The calibration curves of benzoic acid and potassium sorbate were linear by plotting the peak area against the sample concentration over the range from 1.0 mg/L to 400 mg/L. The correlation coefficients of two target compounds were 0.9999 and the concentration limit of detection is 0.15 mg/L. The method was simple, rapid and highly repeatable, and can be used for the determination of benzoic acid and potassium sorbate in drugs.