CAJ | 학술논문

采用响应面法对植物乳杆菌冻干保护剂配比进行优化.在单因素试验的基础上,首先利用Plackett-Burman设计法研究保护剂对响应值的影响程度,发现脱脂乳、甘油和VC对细胞存活率的影响显著,然后利用最陡爬坡法逼近最大响应区域,最后在上升最高点处由中心组合试验和响应面分析确定其最优保护剂复配比例,即脱脂乳、甘油和VC的质量浓度分别为13.68g/100mL、1.97g/100mL和0.20g/100mL,并通过实验测得优化后的细胞存活率为85.01%,与预测值84.85%非常接近,植物乳杆菌含菌量为4.98×10~(10) CFU/g,而加单一保护剂时的细胞存活率最高值为52%,存活率提高了63.48%.
채용향응면법대식물유간균동간보호제배비진행우화.재단인소시험적기출상,수선이용Plackett-Burman설계법연구보호제대향응치적영향정도,발현탈지유、감유화VC대세포존활솔적영향현저,연후이용최두파파법핍근최대향응구역,최후재상승최고점처유중심조합시험화향응면분석학정기최우보호제복배비례,즉탈지유、감유화VC적질량농도분별위13.68g/100mL、1.97g/100mL화0.20g/100mL,병통과실험측득우화후적세포존활솔위85.01%,여예측치84.85%비상접근,식물유간균함균량위4.98×10~(10) CFU/g,이가단일보호제시적세포존활솔최고치위52%,존활솔제고료63.48%.
The formulation of protecant for lyophilized LactobaciUus plantarura was optimized by means of response surface methodology. First, based on the single factor experiments, Plackett-Burman design was used to evaluate the importance of ingredients of compound protectant formula affecting cell viability of Lactobacillus plantarum. Second, steepest ascend method was used to obtain the maximum response regions of the most important ingredients. Finally, central composite design combined with response surface methodology was employed to investigate the optimal compound protectant formula. Results indicated that the most important ingredients included skimmed milk, glycerol and vitamin C, and their optimal amounts in 100 mL of proteetant formula were skimmed milk 13.68 g, glycerol 1.97 g and vitamin C 0.20 g. The optimal cell survival rate was observed to be 85.01% using the optimal compound protectant, which was very close to the expected value (84.85%) and also exhibited an increase of 63.48% compared with using a single proteetant, and the number of living Lactobacillus plantarum was 4.98 ×10~(10) CFU/g.