CAJ | 학술논문

以新鲜缢蛏的碎肉为材料,加水配制成一定比例的匀浆,利用超声波提取法对缢蛏碎肉匀浆进行多糖的提取.利用蒽酮-硫酸法对多糖得率进行计算.将得到的多糖粗品通过DEAE离子交换层析及G-75凝胶层析进一步分离纯化.通过邻苯三酚自氧化体系和Fenton体系测定所得样品的抗氧化能力.结果表明:利用超声波提取多糖的提取率为19.93%.多糖粗品通过DEAE离子交换柱,得到2个峰,峰1对超氧阴离子的清除能率为43.89%,质量浓度为 1 mg/ml时,对·OH清除率为 57.80%.峰2对超氧阴离子的清除能率为57.80%,质量浓度为 1 mg/ml时,对·OH清除率为 77.83%.将DEAE峰2进行G-75凝胶层析得到2个峰,峰1对超氧阴离子的清除能率为83.08%,质量浓度为1 mg/ml时,对·OH清除率为94.76%.峰2对超氧阴离子的清除能率为74.41%,质量浓度为1 mg/ml时,对·OH清除率为52.41%.
이신선액정적쇄육위재료,가수배제성일정비례적균장,이용초성파제취법대액정쇄육균장진행다당적제취.이용은동-류산법대다당득솔진행계산.장득도적다당조품통과DEAE리자교환층석급G-75응효층석진일보분리순화.통과린분삼분자양화체계화Fenton체계측정소득양품적항양화능력.결과표명:이용초성파제취다당적제취솔위19.93%.다당조품통과DEAE리자교환주,득도2개봉,봉1대초양음리자적청제능솔위43.89%,질량농도위 1 mg/ml시,대·OH청제솔위 57.80%.봉2대초양음리자적청제능솔위57.80%,질량농도위 1 mg/ml시,대·OH청제솔위 77.83%.장DEAE봉2진행G-75응효층석득도2개봉,봉1대초양음리자적청제능솔위83.08%,질량농도위1 mg/ml시,대·OH청제솔위94.76%.봉2대초양음리자적청제능솔위74.41%,질량농도위1 mg/ml시,대·OH청제솔위52.41%.
The crude product of polysaccharides was isolated from fresh razor clam Sinonovacula constricta chopper added water by ultrasonic extraction and further purified by DEAE ion-exchange chromatography and Sephadex G-75 chromatography. The antioxidant activity of the polysaccharide substances was determined by fenton system and Pyrogallol oxidation. The results showed that the polysaccharide rate of ultrasonic extraction was 19.93%. The razor clam polysaccharides had effect on scavenging hydroxyl free radicals,and the scavenging hydroxyl free radical ability was increased up to 94.76% and scavenging superoxide anion radical ability was up to 83.08%.