CAJ | 학술논문

结球甘蓝(Brassica oleracea var.capitata)和青花菜(Brassica oleracea var.italica)小孢子胚再生植株频率低是目前影响游离小孢子培养技术有效应用的关键问题之一,研究其小孢子胚植株再生频率的影响因素,提高胚再生植株频率,对促进游离小孢子培养技术在甘蓝类蔬菜育种中更好地应用具有重要意义.该文以结球甘蓝中甘11和青花菜TI-111等基因型为试材.对影响游离小孢子胚再生成植株的固体培养基类型、琼脂浓度、胚的类型及胚在液体培养基中的滞留时间等因素进行了研究.结果表明:游离小孢子培养25天的子叶胚在琼脂浓度为1%-1.25%的B5培养基上植株再生频率最高.进一步通过8个不同基因型对上述实验结果进行了验证,结果显示.游离小孢子培养25天的子叶胚在1%琼脂浓度的B5培养基上植株再生频率达77.8%-97.2%.
결구감람(Brassica oleracea var.capitata)화청화채(Brassica oleracea var.italica)소포자배재생식주빈솔저시목전영향유리소포자배양기술유효응용적관건문제지일,연구기소포자배식주재생빈솔적영향인소,제고배재생식주빈솔,대촉진유리소포자배양기술재감람류소채육충중경호지응용구유중요의의.해문이결구감람중감11화청화채TI-111등기인형위시재.대영향유리소포자배재생성식주적고체배양기류형、경지농도、배적류형급배재액체배양기중적체류시간등인소진행료연구.결과표명:유리소포자배양25천적자협배재경지농도위1%-1.25%적B5배양기상식주재생빈솔최고.진일보통과8개불동기인형대상술실험결과진행료험증,결과현시.유리소포자배양25천적자협배재1%경지농도적B5배양기상식주재생빈솔체77.8%-97.2%.
The application of microspore culture still produces low frequency of plant regeneration from microspore-derived embryos in many cabbage and broccoli genotypes. To improve the frequency of plant regeneration, we studied the effect of medium, agar concentration, embryoid type and days of culture of microspores on plant regeneration in hybrid F_1 generations of a cabbage, 'Zhonggan 11', and a broccoli, TI-111'. B5 medium with 1 %-1.25% agar was optimal for germination and regeneration of embryos, and the frequency of plant regeneration from cotyledonary embryos was significantly higher than that from other embryos, especially cotyledonary embryos from the 25-day-cultured micro-spores. The frequency of plant regeneration for cotyledonary embryos from 25-day-cultured microspores in 8 cultivars of cabbage and broccoli transferred to B5 medium with 1% agar ranged from 77.8% to 97.2%.