CAJ | 학술논문

目的研究重组人白细胞介素-2/ 粒细胞-巨噬细胞集落刺激因子(IL-2/GM-CSF)融合蛋白纯化及复性的最佳条件。方法常规方法提取包涵体,用本室专利技术提纯,复性后样品经DEAE-Sepharose FF离子交换层析,获得融合蛋白。结果获得具有生物活性的融合蛋白,其纯度达到95%。结论此工艺操作简便,纯化效果好,获得率高。
목적연구중조인백세포개소-2/ 립세포-거서세포집락자격인자(IL-2/GM-CSF)융합단백순화급복성적최가조건。방법상규방법제취포함체,용본실전리기술제순,복성후양품경DEAE-Sepharose FF리자교환층석,획득융합단백。결과획득구유생물활성적융합단백,기순도체도95%。결론차공예조작간편,순화효과호,획득솔고。
Objective To study the purification and renaturation of recombinant human IL-2/GM-CSF fusion protein. Methods After the inclusion bodies were isolated, the optimal conditions of denaturation and renaturation were studied. The renatured recombinant human IL-2/GM-CSF was purified by DEAE-Sepharose FF ion-exchange chromatography. Results The purified product manifested biological activity with purity of up to 95%. Conclusions The procedures employed in this study is simple and reliable.