CAJ | 학술논문

目的利用一种新的体外扩增脐带d原始细胞的方法，使得干细胞在数量增加的同时，又能尽量保持原始性。方法在相同细胞因子组合下，用体外液体培养比较脐血有核细胞程序扩增组与一次扩增组及有无基质层条件下的优劣。结果培养28d后，虽然程序扩增组MNC细胞绝对数低于一次扩增组，但其CD34+细胞数明显高于一次扩增组，且有基质层组细胞总数，原始细胞数均高于无基质层组。结论体外程序扩增脐血干细胞是一种有效的扩增干细胞的方法，从而使得脐血的应用更为广泛。
목적이용일충신적체외확증제대d원시세포적방법，사득간세포재수량증가적동시，우능진량보지원시성。방법재상동세포인자조합하，용체외액체배양비교제혈유핵세포정서확증조여일차확증조급유무기질층조건하적우렬。결과배양28d후，수연정서확증조MNC세포절대수저우일차확증조，단기CD34+세포수명현고우일차확증조，차유기질층조세포총수，원시세포수균고우무기질층조。결론체외정서확증제혈간세포시일충유효적확증간세포적방법，종이사득제혈적응용경위엄범。
Objective Using a new method to expand the number of umbilical cord blood stem cells culture in vitro, at the same time to keep their originality. Methods under the same condition with hematopoietic factors in the liquid culture, compared the sequential expansion group and once for all expansion group with or without stroma celllayer. Results When cultured for 28 days, the absolute number of MNC cells in the sequential expansion group was lessthan that in the once for all expansion group,but the number of CD34 + cell in the former was more than that in thelatter. Furthermore, the total number of MNC and progcnitor were greater in that with presence of stromal cell layerthan that with sbsence of stromal cell layer. Conclusion Sequential expansion of umbilical blood stem cells is an effective method for expansion of srem cells which enables more extensive use of umbilical blood. (Shanghai Med J, 2001,24: 95-98)