CAJ | 학술논문

经基因改造的转基因抗虫棉在我国已开始进入大规模商业化应用，对此进行安全性评价是一个十分重要的生态学问题。本文比较了抗虫棉和常规棉中过氧化物酶、多酚氧化酶、酯酶等几种重要同工酶的酶活和酶谱的差异，发现在一定生长期中，抗虫棉的可溶性过氧化物酶活性显著高于常规棉，酯酶的酶活和酶谱在两种棉花中也存在着差异。认为这些差异可能影响抗虫棉的次生代谢，进而导致一些综合的生态学效应，提出植物生理上的改变也是转基因植物安全性评价研究的重要内容。
경기인개조적전기인항충면재아국이개시진입대규모상업화응용，대차진행안전성평개시일개십분중요적생태학문제。본문비교료항충면화상규면중과양화물매、다분양화매、지매등궤충중요동공매적매활화매보적차이，발현재일정생장기중，항충면적가용성과양화물매활성현저고우상규면，지매적매활화매보재량충면화중야존재착차이。인위저사차이가능영향항충면적차생대사，진이도치일사종합적생태학효응，제출식물생리상적개변야시전기인식물안전성평개연구적중요내용。
The transgenic cotton expressing Bacillus thuringiensis (Bt) insecticidal crystal protein is being commercialized in a large scale in China. Several important isoenzymes, including Peroxidases (POD),Polyphenoloxidases (PPO)and Esterases (EST),were compared between two Bt cotton lines and regular cotton. The activity of Peroxidase in Bt cotton was higher than that in regular cotton. There were also diferences between Bt cotton and regular cotton in the Electrophoretic Patterns of Peroxidase and the activity of Esterase isoenzyme. These physiological differences ,maybe caused by genetic manipulation and tissue culture ,could induce changes in secondary metabolism of Bt cotton and then bring ecological effects to environment. This kind of effect is also an important aspect of ecological risk assessment of transgenic plants released into environment.