CAJ | 학술논문

取33日龄高邮鸭和绍兴鸭公鸭各32只，每品种分为两组，分别给以20 h和6 h光照，试验期14 d。试验前后称重。Northern杂交法测定垂体GH mRNA和下丘脑SS mRNA表达水平，放免法测定血清中生长激素(GH)、胰岛素生长因子-1(IGF-1)、甲状腺激素(T3、T4）水平。结果表明：高邮鸭20 h光照时增重、GH及T4水平显著低于6 h光照(P<0.01)；而绍兴鸭的增重变化则与高邮鸭相反(P<0.01)，血浆GH水平变化与高邮鸭相同，T4水平无显著变化。光照制度的改变未引起两品种鸭IGF-1、T3水平的显著变化。垂体GH mRNA表达水平的变化与增重变化相反。提示光照直接影响鸭生长轴基因的表达以及激素水平，从而显著影响生长速度，但这种影响在不同品种可有完全不同的表现。
취33일령고유압화소흥압공압각32지，매품충분위량조，분별급이20 h화6 h광조，시험기14 d。시험전후칭중。Northern잡교법측정수체GH mRNA화하구뇌SS mRNA표체수평，방면법측정혈청중생장격소(GH)、이도소생장인자-1(IGF-1)、갑상선격소(T3、T4）수평。결과표명：고유압20 h광조시증중、GH급T4수평현저저우6 h광조(P<0.01)；이소흥압적증중변화칙여고유압상반(P<0.01)，혈장GH수평변화여고유압상동，T4수평무현저변화。광조제도적개변미인기량품충압IGF-1、T3수평적현저변화。수체GH mRNA표체수평적변화여증중변화상반。제시광조직접영향압생장축기인적표체이급격소수평，종이현저영향생장속도，단저충영향재불동품충가유완전불동적표현。
Thirty-three day-old Gaoyou and Shaoxing male ducks were used in the present study.32 birds from each breed were divided into 2 groups each,with one group being exposed to 20 h lighting per day(LL),and another 6 h per day(SL)for 2 weeks.SS mRNA of hypothalama and GH mRNA of pituitaries were quantified by Northern blotting hybridization.GH,IGF-1，T３ and T４ were determined by RIA.The results showed that liveweight gain,GH and T４ of LL treated Gaoyou ducks were significantly lower than those of SL treated （P<0.01)，while liveweight gain of Shaoxing ducks was significantly higher in LL treated than that in SL treated(P<0.01)，the alteration of GH levels appeared the same as in Gaoyou ducks and T４ did not vary significantly.No significant differences of IGF-1 and T３ levels were found between SL and LL groups in both breeds.The effects of light regimen on pituitary GH mRNA expression were opposite to that on liveweight gain in both breeds.This suggests that lighting regimen directly affect hormonal levels and gene expression of somatotropic axis which further influences liveweight gain.The effects of lighting regimen on growth performance as well as gene expression can be totally different in different breeds of ducks.