CAJ | 학술논문

目的研究细胞角蛋白7(CK 7)、癌胚抗原(CEA)和细胞角蛋白20(CK 20)单克隆抗体在卵巢和胃粘液癌中的表达及鉴别意义。方法采用免疫组织化学S-P法对各组癌进行3种单克隆抗体表达的检测。结果二组间各种抗体染色有一定的重叠。8例(1*)来源于卵巢的粘液癌CK 7均呈阳性表达“++”～“+++”，CEA 3/8不同程度表达，CK 20 7/8阴性，经实验结果证实其中1*例应是原发于卵巢；18例胃来源的粘液癌CK 7、CEA和CK 20皆不同程度阳性。经t检验，CK 7和CEA表达在两组间有显著差异(P<0.05)，而CK 20在两组间表达无显著差异(P>0.05)。结论单一CK 7、CEA和CK 20不能有效鉴别来自胃和卵巢的粘液癌。CK 7+CEA+CK 20联合是鉴别卵巢来源和胃来源粘液癌最有效的组合。
목적연구세포각단백7(CK 7)、암배항원(CEA)화세포각단백20(CK 20)단극륭항체재란소화위점액암중적표체급감별의의。방법채용면역조직화학S-P법대각조암진행3충단극륭항체표체적검측。결과이조간각충항체염색유일정적중첩。8례(1*)래원우란소적점액암CK 7균정양성표체“++”～“+++”，CEA 3/8불동정도표체，CK 20 7/8음성，경실험결과증실기중1*례응시원발우란소；18례위래원적점액암CK 7、CEA화CK 20개불동정도양성。경t검험，CK 7화CEA표체재량조간유현저차이(P<0.05)，이CK 20재량조간표체무현저차이(P>0.05)。결론단일CK 7、CEA화CK 20불능유효감별래자위화란소적점액암。CK 7+CEA+CK 20연합시감별란소래원화위래원점액암최유효적조합。
Objective　To discriminate if the mucinous carcinomas are primary to the ovary,stomach or metastases from ovary and stomach by monoclonal antibodies cytokeratin 7,carcinoembryonic antigen,and cytokeratin 20.Methods　Immunohistochemical methods were used with these monoclonal antibodies and S-P kit.Results　The immunostaing patterns of CK 7,CEA,and CK 20 showed a considerable overlap between the two types of mucinous carinomas,8 cases of ovarian mucinous carcinomas were always positive for CK 7 and a lesser extent for CEA,18 cases of gastric mucinous carcinomas showed a different degree positivity for CK 7,CEA,and CK 20.In discriminant analysis,three antibodies (CK 7,CEA,CK 20) appeared to be necessary for optimal classification,7/8 ovarian mucinous carcinomas and 10/18 gastric mucinous carcinomas were classified correctly,and 1 case of gastric mucinous carcinoma should originate from ovary.The difference of CK 7 and CEA between the two groups was significant(P<0.05),but the difference of CK 20 between the two groups was not significant(P>0.05).Conclusion　One of CK 7,CEA,and CK 20 may not differentiate between the two groups.The combination of CK 7,CEA,and CK 20 is sufficient for classification in most mucinous carcinomas originated to stomach or ovary.