CAJ | 학술논문

转双抗虫基因741杨特征及其繁殖技术
전쌍항충기인741양특정급기번식기술
THE CHARACTERISTIC OF TWO INSECT-RESISTANT GENES TRANSFORMED HYBRID POPLAR 741 AND ITS PROPAGATION METHODS

万方数据

河北林果研究河北林果研究 하북림과연구
HEBEI JOURNAL OF FORESTRY AND ORCHARD RESEARCH
2001年 2期 97-117 ,共21页

郑均宝%裴保华%梁海永%王进茂%王永芳%杜克久%杨敏生%高宝嘉%田颖川%贾燕涛%虞红梅%马玉湖%马玉行

정균보%배보화%량해영%왕진무%왕영방%두극구%양민생%고보가%전영천%가연도%우홍매%마옥호%마옥행

双抗虫基因%741杨%转化雙抗蟲基因%741楊%轉化
쌍항충기인%741양%전화

简述了当今植物抗虫基因工程的研究进展，介绍了Bt基因和蛋白酶抑制剂基因，农杆菌介导的遗传转化以及防治害虫产生抗性的策略等。双抗虫基因是用部分改造BtCry1Ac基因和慈菇蛋白酶抑制剂基因（API）构建的植物表达载体。被转化体741杨（Populus×aldatomentosa Cl. 741）是以银白杨×（山杨＋小叶杨）为母本，毛白杨为父本，1994年杂交育成，为一优良白杨无性系，形态酷似毛白杨。在转双抗虫基因的研究中，选出高抗虫、中抗虫无性系。高抗虫无性系杀死昆虫幼虫的总死亡率为83％～90％。从苗期高生长和形态观察，转双抗虫基因741杨生长发育正常。比较详细地介绍了741杨营养繁殖技术及基本原理。介绍了硬枝扦插容器育苗、组培微繁技术、容器育苗的移栽和苗圃管理以及采穗圃的建立和经营等。
간술료당금식물항충기인공정적연구진전，개소료Bt기인화단백매억제제기인，농간균개도적유전전화이급방치해충산생항성적책략등。쌍항충기인시용부분개조BtCry1Ac기인화자고단백매억제제기인（API）구건적식물표체재체。피전화체741양（Populus×aldatomentosa Cl. 741）시이은백양×（산양＋소협양）위모본，모백양위부본，1994년잡교육성，위일우량백양무성계，형태혹사모백양。재전쌍항충기인적연구중，선출고항충、중항충무성계。고항충무성계살사곤충유충적총사망솔위83％～90％。종묘기고생장화형태관찰，전쌍항충기인741양생장발육정상。비교상세지개소료741양영양번식기술급기본원리。개소료경지천삽용기육묘、조배미번기술、용기육묘적이재화묘포관리이급채수포적건립화경영등。
We reviewed briefly the progress of genetic engineering on insect-resistant plants, especially on the Bt gene, the API gene, the agrobacterium-mediated transformation method and the ideas of preventing the resistance formation of insects. The expression vector pBtiA containing the two insect-resistant genes, BtCry1Ac gene and API gene, was introduced into the hybrid poplar 741（Populus×aldatomentosa Cl.741）,which was obtained in 1994 by hybridizing P.alba L.×(P.davidianaDode+P.simonii Carr.) with P.tomentosa Carr.. The hybrid poplar 741 was a good clone. Its characteristics are very similar to that of P.tomentosa Carr.. The high insect-resistant clone and the middle insect-resistant clone were selected in insect feeding tests. The larvae mortality rate of the high insect-resistant clone was 83%～90%. The gene-transformed hybrid poplar 741 expressed normally on morphology during its seedling phase. The asexual propagation including cutting, tissue culture, and related principles and skills were attentively introduced in the paper.